Dow Agrosciences LLC

Sangamo Biosciences, Inc.

Sangamo Therapeutics, Inc.

Jon Christopher Mitchell

Patricia C Apel-Birkhold

Timothy Denver Hey

Thomas Meade

Scott Bevan

Scott B Bintrim

Jeffrey C Miller

Fyodor Urnov

Justin Michael Lira

Ignacio Mario Larrinua

Sarah E Worden

Joel Jay Sheets

Joseph F Petolino

Sean Michael Russell

Nicole Arnold

Lining Guo

Vipula Shukla

Ryan C Blue

Scott A Young

Qihua C Cai

Robbi J Garrison

Kristin J Fencil

Robin Leola Thompson

Ze-Sheng Li

Lisa W Baker

Donald J Merlo

Baolong Zhu

Gary David Gustafson

Clive Waldron

Barry W Schafer

Susan B Green

Jeffrey A Miller

Weiting W Ni

Donald R Hahn

Amanda D Schleper

Ze Sheng Li

Nicole L Arnold

Vipula K Shukla

Jeffrey Miller

Brian S Bullard

James D Jackson

Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.

Optimized non-canonical zinc finger proteins

The invention relates to isolated genes encodingtoxin complex (TC) proteins, and transgenic plants and host cells expressingtoxin complex (TC) proteins and having resistance to insects.

Genes encoding toxin complex proteins and uses thereof

The subject invention relates to noveltoxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable fromstrain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.

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The subject invention relates to noveltoxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable fromstrain ILM104.

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable fromandcan be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a 'stand-alone' TC protein (fromorfor example) is enhanced by one or more TC protein 'potentiators' derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-aloneprotein toxin as well as a stand-aloneprotein toxin. (As one skilled in the art knows,toxin proteins tend to be more desirable for controlling lepidopterans whiletoxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

Mixing and matching TC proteins for pest control

The subject invention relates to noveltoxin complex (TC) proteins encoded by genes fromstrain ILM 104, and methods of controlling an insect with the toxin proteins.

The subject invention relates to novel nucleic acid encoding astrain Xwi toxin complex (TC) protein and plants and bacteria transformed therewith.

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West Lafayette, IN, United States of America

Jon Christopher Mitchell