Pioneer Hi-Bred International, Inc.

Jon Aaron Tucker Reinders

William James Gordon-Kamm

Keith S Lowe

Huaxun Ye

Tim Fox

Oleksandr Dovzhenko

Maja Temerinac-ott

Olaf Tietz

Rachel Carol Huegel

Sean Walsh

Cristina Dal Bosco

The methods have application to the tissue culture of plant cells, ovules and microspores. Automated imaging, automated cell tracking and predictive modeling are used to develop methods that predict the likelihood that a plant cell will develop into a desired phenotype and/or which cellular reprogramming factors will assist in this development. The methods taught herein can also be used to evaluate the toxicity effects of compounds on plant cells, predict genotypic responses to tissue culture and cellular reprogramming factors, determine cell ploidy status, and predict other types of cell phenotype development.

Microspore culture predictive model generation and use

The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.

Methods for clonal plant production

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Systems and methods for cellular reprogramming of a plant cell

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Clive, IA, United States of America

Jon Aaron Tucker Reinders