Life Technologies Corporation

Molecular Probes, Inc.

Jolene Ann Bradford

Stephen T Yue

Jixiang Liu

Shih-Jung Huang

Gayle Buller

Ching-Ying Cheung

Patrick Pinson

Kyle R Gee

Hee Chol Kang

Vladimir Martin

Michael Wayne Olszowy

Christopher Langsdorf

Gregory Kaduchak

Michael D Ward

Cody Collier Henderson

Justin Shepherd Hicks

James Kolozs

Phillip Nolan Tyler

Sean Michael Cole

Scott Clarke

William Lunt Godfrey

Jan Zwierstra

Ian Bradley Thorne

Robin Carlton Swain

David Anton Van Gogh

Brandon J Burlington

Yusuf Shirazi

Patrick R Pinson

Michael Dennis Ward

Andrew Thomas George Parker

Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

7-hydroxycoumarin-based cell-tracking reagents

The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

Dual labeling methods for measuring cellular proliferation

Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

Anthraquinone based near IR emitting compounds and uses thereof

The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

Violet laser excitable dyes and their method of use

Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use

The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a &#x2014;N,N,O-triacetic acid analog of BAPTA and has the following formula 

Heavy metal binding compounds and their method of use

Actinomysin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided. The actinomysin-based near IR emitting compounds have the structure:  wherein R is H or NH; R, R, R, and Rare independently a moiety comprising 1-30 atoms selected from H, O, C, and N, wherein the atoms are in a linear, branched, or cyclic configuration; Rand/or Rcomprise a quaternary nitrogen atom; and Ris H, F, or Cl.

Modified actinomycin-based nucleic acid stains and methods of their use

Cytometer

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Eugene, OR, United States of America

Jolene Ann Bradford