Battelle Memorial Institute

International Paper Company

Catchlight Energy LLC

Johnway Gao

Rodney S Skeen

Brian S Hooker

Daniel B Anderson

Jerome U Gilberry

John J Kester

Clark A Morrow

Amar N Neogi

Andrew P Bartko

Ziyu Dai

Jeffrey P Carpenter

Rodney S Black

Lifang Shi

William A Ivancic

Jason A Schaefer

Joseph A McClintock

Benjamin Levie

Dwight Anderson

William A Ivanic

Methods of producing clean (e.g., low sulfur and metal ion content, and free of fermentation inhibitors) sugar and lignin-rich streams, and downstream conversion products, from lignocellulosic biomass, may include obtaining non-chemically pretreated, milled lignocellulosic biomass, reacting the milled lignocellulosic biomass with an enzymatic agent to produce a slurry that includes converted monomeric lignocellulosic sugars and lignin-rich residuals, and separating the slurry into a sugar stream that includes the converted monomeric lignocellulosic sugars and a lignin-rich stream that includes the lignin-rich residuals. The sugar stream, not including water, includes at least 75% monomeric lignocellulosic sugar, less than 0.20% sulfur, and less than 3.0% metal ion content, and the lignin-rich stream includes at least 35% lignin and less than 0.50% sulfur. Some methods include producing fermentation products such as alcohols and/or organic acids from the sugar stream, and/or use of the lignin residuals in fuels.

Clean sugar and lignin from non-chemically pretreated lignocellulosic biomass

A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.

Sample preparation for spectroscopy analysis

The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.

Methods of spraying saccharification enzymes and fermentation organisms onto lignocellulosic biomass for hydrolysis and fermentation processes

A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.

Biological and chemical monitoring

Systems and methods for biological and chemical detection

Optical devices for biological and chemical detection

The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strainThe isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Isolated yeast promoter sequence and a method of regulated heterologous expression

The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.

Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco

The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Method for using a yeast alpha-amylase promoter

Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, . The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in

Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in

Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

The present invention is a pulse spilling self-aerator (PSSA) that has the potential to greatly lower the installation, operation, and maintenance cost associated with aerating and mixing aqueous solutions. Currently, large quantities of low-pressure air are required in aeration systems to support many biochemical production processes and wastewater treatment plants. Oxygen is traditionally supplied and mixed by a compressor or blower and a mechanical agitator. These systems have high-energy requirements and high installation and maintenance costs. The PSSA provides a mixing and aeration capability that can increase operational efficiency and reduce overall cost.

Growing community of inventors

Richland, WA, United States of America

Johnway Gao