Cytonome/st, LLC

Inguran, LLC

St Reproductive Technologies, LLC

Xy, Inc.

Dako Denmark A/s

Dako Colorado, Inc.

Johnathan Charles Sharpe

Donald Francis Perrault, Jr

Blair D Morad

Nemanya Sedoglavich

Emanuel Tito Mendes Machado

Kenneth Michael Evans

Kris S Buchanan

Erin Koksal

Kristopher Scott Buchanan

Rudolf Hulspas

Ramakrishnan Vishwanath

Juan F Moreno

Thomas Boyd Gilligan

Paul Julian Martinsen

Philip Rowe

Kris Buchanan

Richard W Lenz

Vladimir Shapiro

Kiryakos Spiro Mutafopulos

Stuart R Ehrlich

Donald Francis Perrault

Peter Kiesel

Jeffrey T Samson

James V Osburn

Peter Niven Schaare

Sam Hogue

Michael I Morkos

Megan Lynne Sauter

Sean B Pieper

Stuart Buddy Ehrlich

Martin Joe Costello

Blair Morad

Donald F Perrault

Donald Perrault

Donald Frances Perrault

A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region.

Flow cell

This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

Systems for high throughput sperm sorting

A method, system and apparatus for anti-sorting particles is disclosed. In an example embodiment, particles move in a fluid along a microfluidic channel. A measurement device determines which particles are selected or desired particles by measuring a desired characteristic of the particles and outputting an associated signal. The energy source continuously imparts a force on unselected or undesired particles in a deflection region of the microfluidic channel to remove the unselected or undesired particles from the stream of particles. Based upon the signal outputted by the measurement device, the energy source is controlled to reduce or eliminate the force on selected particles such that the selected particles flow on a natural or expected flow path to a collection area such as an output channel. By avoiding application of force to desired particles, the anti-sorting systems and methods can improve viability and other characteristics of the desired particles.

Method and apparatus for an anti-sorting flow cytometer

Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

Edge chip

A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.

Hydrodynamic focusing apparatus and methods

Methods for high throughput sperm sorting

Systems and methods taught herein advantageously provide extended dynamic range capabilities to detect low intensity and high intensity emitted or scattered light from particles at high speeds with high sensitivity. Independently controlled first and second optical detector elements that handle light intensities in different dynamic ranges, large overall dynamic range is created. Signals from the detector elements can be combined to create a single combined signal that has excellent sensitivity over a large dynamic range. The detector systems and methods taught herein are particularly advantageous in particle processing where the population of particles can emit or scatter light over a large range of intensity values. Systems and methods taught herein enable a wide dynamic range, optical signals of related to particles of interest within a single detector's dynamic range can be acquired while other optical signals at light intensities outside the single detector's dynamic range can also be accurately captured.

Systems and methods for extended dynamic range detection of light

A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

Thermal activated microfluidic switching

The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles).

Micro-lens systems for particle processing systems

A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.

Nozzle assembly for a flow cytometry system and methods of manufacture

Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0&#xb0;) and side detection (i.e., 90&#xb0;). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.

Assemblies and methods for reducing optical crosstalk in particle processing systems

Growing community of inventors

Hamilton, New Zealand

Johnathan Charles Sharpe