Johnson & Johnson Clinical Diagnostics, Inc.

John William H Sutherland

John W Backus

William Harold Donish

John Bruce Findley

Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure. This procedure includes a series of steps which have critically defined temperature and time parameters. Each polymerase chain reaction cycle requires generally less than about two minutes, and in most cases less than 90 seconds. At least 5 units/100 &mgr;l of solution of thermostable DNA polymerase are used, and other preferred levels of primer concentrations facilitate the quick cycling in the amplification. In preferred embodiments, only two temperatures are used in the amplification.

Growing community of inventors

Rochester, NY, United States of America

John William H Sutherland