Beckman Coulter, Inc.

John Warwick Silzel

Robert J Obremski

Yagang Liu

Ronald J Schoengold

Charles L Dodson

Richard Sergei Matusewicz

Tsong-Tseh Tsay

Wayne H Schrier

Tung Rung Wang

Bibijana Cercek

Zhou Shaomin

A microscale binding assay, analyte binding array, and kits are disclosed, which exploit the mass action law to harvest analyte from a liquid sample. This is achieved by fabrication of sorbent zones having up to ten times the binding capacity per unit area generally obtained on polystyrene microtiter plates. The resulting arrays substantially deplete the liquid solution of analyte during incubation. Accordingly, the assays respond to total mass of analyte in the sample, not analyte concentration. This approach, coupled with direct fluorescence detection in the NIR, yields maximal signal intensity and low background for optimal sensitivity.

Detection of very low quantities of analyte bound to a solid phase

A medical analysis system for reading and interpreting an occult blood test (OBT) device is provided. The instant system comprises an image sensor for capturing an image of the entire test area of the fecal occult blood test (FOBT) device and for converting the image into a digital data. The system further comprises a data processor conventionally coupled to the image sensor. The data processor compares the digital data with pre-determined threshold conditions of a positive FOBT by utilizing a spectrographic and morphologic image analysis algorithm and determines a presence of the occult blood in the sample. A hue angle analysis for data processing may be used. A method of performing FOBT on a sample is also provided.

Devices and methods for reading and interpreting guaiac-based occult blood tests

A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having a planar surface with a plurality of probes of known recognition to the target analytes thereon. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the planar surface of the waveguide at a plurality of different locations, by causing relative movement between the waveguide and the laser light, so that evanescent waves radiate from the waveguide. Where conjugate has attached to a probe, there is emission of light different from that emitted by a probe without conjugate attached thereto.

System for simultaneously conducting multiple ligand binding assays

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Yorba Linda, CA, United States of America

John Warwick Silzel