Scarab Genomics, LLC

Wisconsin Alumni Research Foundation

Campbell, John W.

John Walter Campbell

Frederick R Blattner

Guy Plunkett

Gyorgy Posfai

David A Frisch

Charles Landry

Valerie Burland

Nian Shi

Buffy Stahl

György Posfai

Reduced genome strains ofMGl 655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.

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The present invention relates to novel strains of microorganisms with oxygen-regulated metabolism. The microorganisms have higher growth rates and are more efficient than parental strains. The microorganisms may be used to produce a variety of products of interests, such as recombinant proteins, nucleic acids, such as DNA, amino acids, and chemicals.

Oxygen-regulated microorganisms

The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Prophage element-free bacteria

Reduced genome strains ofMG1655 are described. In various embodiments, the strains have one or more of equal or improved growth rate, transformation efficiency, protein expression, DNA production, DNA yield and/or DNA quality compared to the parental strain and commercially available strains.

A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.

Insertion sequence-free bacteria

The invention relates to host cells with improved protein expressed properties. The host cells comprise rare tRNA genes within the one or more rRNA operons.

Methods and compositions for producing recombinant proteins using a gene for TRNA

The various embodiments of the present invention relate generally to plasmid DNA preparations and to methods for producing and using such preparations.

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Oak Park, IL, United States of America

John Walter Campbell