Caliper Technologies Corporation

Molecular Devices Corporation

Caliper Life Sciences, Inc.

Mountain View

John Wallace Parce

Robert S Dubrow

Anne R Kopf-Sill

Luc J Bousse

Michael R Knapp

Harden M McConnell

Calvin Y H Chow

Gillian M Humphries

Andrea W Chow

Calvin Y Chow

Robert F Zuk

Colin B Kennedy

Steven A Sundberg

Dean G Hafeman

John C Owicki

Richard J McReynolds

Josef E Kercso

Karen M Kercso

Claudia B Cohen

Jonathan Briggs

Claudia B Jaffe

Christopher Bloxsom

Steven A Sunberg

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.

High throughput screening assay systems in microscale fluidic devices

Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components and drug screening are provided. Microfluidic devices, systems and methods for using these devices and systems for performing a wide variety of fluid operations are provided. The devices and systems of are used in performing fluid operations which require a large number of iterative, successive or parallel fluid manipulations, in a microscale, or sealed and readily automated format.

Microfluidic sequencing systems

Microfluidic sequencing methods

Microfluidic devices are provided for the performance of chemical and biochemical analyzes, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.

Controlled fluid transport in microfabricated polymeric substrates

Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.

Apparatus and methods for correcting for variable velocity in microfluidic systems

Microfluidic device for sequencing by hybridization

Apparatus and method for correcting for variable velocity in microfluidic systems

The present invention is generally directed to improved methods, structures and systems for interfacing microfluidic devices with ancillary systems that are used in conjunction with such devices. These systems typically include control and monitoring systems for controlling the performance of the processes carried out within the device, e.g., controlling internal fluid transport and direction, monitoring and controlling environmental conditions and monitoring results of the processes performed, e.g., detection.

Controller/detector interfaces for microfluidic systems

The present invention generally provides a micropump that utilizes electroosmotic pumping of fluid in one channel or region to generate a pressure based flow of material in a connected channel, where the connected channel has substantially no electroosmotic flow generated. Such pumps have a variety of applications, and are particularly useful in those situations where the application for which the pump is to be used prohibits the application of electric fields to the channel in which fluid flow is desired, or where pressure based flow is particularly desirable.

Micropump

The present invention is generally directed to improved microfluidic devices, systems and methods of using same, which incorporate channel profiles that impart significant benefits over previously described systems. In particular, the presently described devices and systems employ channels having, at least in part, depths that are varied over those which have been previously described. These varied channel depths provide numerous beneficial and unexpected results.

Microfluidic systems incorporating varied channel dimensions

Microfluidic devices are provided for the performance of chemical and biochemical analyses, syntheses and detection. The devices of the invention combine precise fluidic control systems with microfabricated polymeric substrates to provide accurate, low cost miniaturized analytical devices that have broad applications in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields.

The present invention is generally directed to methods, apparatus and systems for use in performing in situ dilution or concentration of a particular subject material in a microfluidic device or system. These methods and apparatus may generally be integrated with other microfluidic operations and/or systems, to perform a number of different manipulations, wherein dilution or concentration, carried out within the context of the microfluidic device or system, is just one part.

Method for in situ concentration and/or dilution of materials in microfluidic systems

The present invention generally provides microfluidic devices ( ) and systems that utilize electrokinetic material transport systems to selectively control and direct the transport of materials through and among complex arrangements of integrated microscale channels (for example, and &plus;2n, where n&equals;0, 1, . . . 8) disposed within integrated body structures.

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Palo Alto, CA, United States of America

John Wallace Parce