The University of Melbourne

Csl Limited

Other

Zoetis Services LLC

Commonwealth Scientific and Industrial Research Organisation

Walter and Eliza Hall Institute of Medical Research

The Council of Queensland Institute of Medical Research

Csi Limited

John Walker

David Charles Jackson

Souravi Ghosh

Vernon Morrison Bowles

Mark Douglas Gorrell

Elza Nicole Theresia Meeusen

Rogan Lee

Stephen William Doughty

Malcolm Roy Brandon

An adjuvant composition which comprises an anionic macromolecule component particularly an ionic polysaccharide such as DEAE-dextran, and a saponin component, particularly an immunostimulating complex component. Immunogenic compositions comprising an immunogen and this adjuvant composition are also disclosed together with methods of use thereof.

Saponin adjuvant compositions and methods relating thereto

The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of SSKTQTHTQQDRPPQPS (SEQ ID NO: 1); QPSTELEETRTSRARHS (SEQ ID NO: 2); RHSTTSAQRSTHYDPRT (SEQ ID NO: 3); PRTSDRPVSYTMNRTRS (SEQ ID NO: 4); TRSRKQTSHRLKNIPVH (SEQ ID NO: 5); SHQYLVIKLIPNASLIE (SEQ ID NO: 6); IGTDNVHYKIMTRPSHQ (SEQ ID NO: 7); YKIMTRPSHQYLVIKLI (SEQ ID NO: 8); KLIPNASLIENCTKAEL (SEQ ID NO: 9); AELGEYEKLLNSVLEPI (SEQ ID NO: 10); KLLNSVLEPINQALTLM (SEQ ID NO: 11); EPINQALTLMTKNVKPL (SEQ ID NO: 12); FAGVVLAGVALGVATAA (SEQ ID NO: 13); GVALGVATAAQITAGIA (SEQ ID NO: 14); TAAQITAGIALHQSNLN (SEQ ID NO: 15); GIALHQSNLNAQAIQSL (SEQ ID NO: 16); NLNAQAIQSLRTSLEQS (SEQ ID NO: 17); QSLRTSLEQSNKAIEEI (SEQ ID NO: 18); EQSNKAIEEIREATQET (SEQ ID NO: 19); TELLSIFGPSLRDPISA (SEQ ID NO: 20); PRYIATNGYLISNFDES (SEQ ID NO: 21); CIRGDTSSCARTLVSGT (SEQ ID NO: 22); DESSCVFVSESAICSQN (SEQ ID NO: 23); TSTIINQSPDKLLTFIA (SEQ ID NO: 24), SPDKLLTFIASDTCPLV (SEQ ID NO: 25) and SGRRQRRFAGVVLAGVA (SEQ ID NO: 26).

T helper cell epitopes

The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of EPINQALTLMTKNVKPL (SEQ ID NO: 12); FAGVVLAGVALGVATAA (SEQ ID NO: 13); NLNAQAIQSLRTSLEQS (SEQ ID NO: 17) and TELLSIFGPSLRDPISA (SEQ ID NO: 20).

The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of PRTSDRPVSYTMNRTRS (SEQ ID NO: 4); TRSRKQTSHRLKNIPVH (SEQ ID NO: 5); SHQYLVIKLIPNASLIE (SEQ ID NO: 6); and SPDKLLTFIASDTCPLV (SEQ ID NO: 25).

An isolated antigen against a, prepared by a method including providing a sample of aand an antibody probe, probing thesample with the antibody probe to detect at least one antigen, and isolating the antigen detected. The antibody probe includes at least one antibody against thethat is produced by a method including (a) providing a biological sample taken a short time after an immune animal has been challenged with aorextract taken from the infection site or an area of a lesion or an area close to the infection site or lesion; (b) isolating cells from the biological sample; (c) culturing the cells in vitro in a suitable culture medium; and (d) harvesting antibodies produced from the cell.

Antigen composition against mycoplasma

The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of SSKTQTHTQQDRPPQPS (SEQ ID NO: 1); QPSTELEETRTSRARHS (SEQ ID NO: 2); QSLRTSLEQSNKAIEEI (SEQ ID NO: 18); and DESSCVFVSESAICSQN (SEQ ID NO: 23).

The present invention provides T helper cells epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of SSKTQTHTQQDRPPQPS (SEQ ID NO:1); QPSTELEETRTSRARHS (SEQ ID NO:2); RHSTTSAQRSTHYDPRT (SEQ ID NO:3); PRTSDRPVSYTMNRTRS (SEQ ID NO:4); TRSRKQTSHRLKNIPVH (SEQ ID NO:5); SHQYLVIKLIPNASLIE (SEQ ID NO:6); IGTDNVHYKIMTRPSHQ (SEQ ID NO:7); YKIMTRPSHQYLVIKLI (SEQ ID NO:8); KLIPNASLIENCTKAEL (SEQ ID NO:9); AELGEYEKLLNSVLEPI (SEQ ID NO:10); KLLNSVLEPINQALTLM (SEQ ID NO:11); EPINQALTLMTKNVKPL (SEQ ID NO:12); FAGVVLAGVALGVATAA (SEQ ID NO:13); GVALGVATAAQITAGIA (SEQ ID NO:14); TMQITAGIALHQSNLN (SEQ ID NO:15); GIALHQSNLNAQAIQSL (SEQ ID NO:16); NLNAQAIQSLRTSLEQS (SEQ ID NO:17); QSLRTSLEQSNKAIEEI (SEQ ID NO:18); EQSNKAIEEIREATQET (SEQ ID NO:19); TELLSIFGPSLRDPISA (SEQ ID NO:20); PRYIATNGYLISNFDES (SEQ ID NO:21); CIRGDTSSCARTLVSGT (SEQ ID NO:22); DESSCVFVSESAICSQN (SEQ ID NO:23); TSTIINQSPDKLLTFIA (SEQ ID NO:24); SPDKLLTFIASDTCPLV (SEQ ID NO:25) and SGRRQRRFAGVVLAGVA (SEQ ID NO:26).

Methods and kits for identifying antigens from a pathogen. The antigens identified are preferably those that provide protection for an animal against the pathogen. The steps of the method include providing a sample of the pathogen, isolating the protective antigen from the sample of antigen with an antibody probe. The antibody probe is produced by obtaining a biological sample containing antibody producing cells from an immune animal infected with or challenged by the pathogen or pathogen extract, and obtaining the antibodies from these cells by culturing the cells. These methods and kits are useful for preparing antigens to be used as vaccines, in diagnostic kits, and to prepare polyclonal or monoclonal antibodies.

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Victoria, Australia

John Walker