Abbott Laboratories Corporation

Idexx Laboratories, Inc.

Iris International, Inc.

Abbott Point of Care Inc.

John W Roche

Richard G Kendall

W Peter Hansen

Bart Jan Wanders

Thomas H Adams

Harold C Flynn, Jr

Harvey Lee Kasdan

Harold Richardson Crews

Michelle L Coleman

Harvey L Kasdan

Niten V Lalpuria

Darryn W Unfricht

James Russell

Igor Nikonorov

Vu Phan

Benjamin Ports

Kyle Hukari

Douglas R Olson

Peter W Hansen

Ralph W Dowdell, Iii

Marcus F Julian

Robin A Levy

James W Russell

Harold C Flynn

For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.

Dynamic range extension systems and methods for particle analysis in blood samples

A method for counting blood cells in a sample of whole blood. The method comprises the steps of:

Method for performing a blood count and determining the morphology of a blood smear

A method for counting blood cells in a sample of whole blood. The method comprises the steps of: Optionally, steps of fixing and staining of blood cells on the slide can be employed in the method.

A biological fluid sample analysis cartridge, analysis system, and method for analyzing a biologic fluid sample are provided. The cartridge includes a collection port, at least one channel within the cartridge in fluid communication with the collection port, a passage in fluid communication with the at least one channel, and an analysis chamber mounted on a tray. The tray is mounted relative to the cartridge and selectively positionable relative to the passage in a first position where the analysis chamber will engage a bolus of sample extending out from the passage to permit selective transfer of sample from the bolus to the analysis chamber.

Biologic fluid sample analysis cartridge

The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses. The consumable tubes incorporate reference particles, which act as internal standards to ensure that the dilutions made during processing of the samples have been carried out correctly, and to ensure that the instrument is working properly. The present invention also relates to methods for using the system.

Consumable tube for use with a flow cytometry-based hematology system

A hydraulic system for a flow cytometry having a reservoir for a sheath solution, at least one syringe connected to the reservoir, the at least one syringe being driven by a motor, a single HGB module connected on an inlet side to a sample inlet which is connected to the flow cell by a single flow path passing through the HGB module, a single flow cell connected to the HGB module and at least one syringe on an inlet side of the flow cell, and to a waste container on an outlet side of said flow cell.

Flow-cytometry-based hematology system

Flow cytometry-based hematology system

The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthogonally to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input (&ldquo;right angle scatter&rdquo;). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90&deg;. This scatter is termed forward scatter light&rdquo; and is collected on two distinct photo detectors, that represent 'forward scatter low' (FSL) which has an angle of from about 1&deg; to about 3&deg;, and 'forward scatter high' (FSH) which has an angle of from about 9&deg; to about 12&deg; from the orientation of the original beam from laser input. A third photo detector is placed in between these two forward scatter detectors, that is axial with the impinging laser light. This detector measures axial light loss, or light extinction (EXT) which is the sum of all the light that is absorbed and scattered by the blood cells. A right angle scatter light detector is oriented to receive the previously mentioned right angle scatter light. A forward scatter light detector is oriented to capture the previously mentioned forward scatter light oriented different angles from the beam of the laser input.

High numerical aperture flow cytometer and method of using same

Growing community of inventors

Scarborough, ME, United States of America

John W Roche