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University of Florida Research Foundation Inc

John W Guy

Nicholas Muzyczka

Nucleic acid mitochondrial delivery systems are provided. These systems are important for delivery of genetic information to a mitochondrion and for treatment of mitochondrial related diseases.

Mitochondrial nucleic acid delivery systems

A synthetic, nuclear-encoded ND4 gene was linked to a mitochondrial targeting sequence and a FLAG epitope tag. This fusion construct was inserted into a rAAV vector. The ND4 fusion protein was expressed and imported into the mitochondria of cells harboring a mitochondrial DNA mutation (G11778A), where it restored cellular respiration.

Reducing cellular dysfunction caused by mitochondrial gene mutations

A carpet measurement tool comprising a rigid rectangular plate having an upper surface, a lower surface, a pair of opposed long edges, and a pair of short edges extended therebetween; a distance ruler with a set length and scaling disposed on the upper surface of the plate near one long edge, the distance ruler a plurality of equally spaced tick marks disposed thereon; a composite ruler having a scaling equal to the distance ruler and disposed near the other long edge, the composite ruler including a plurality of sub-rulers positioned in a stacked relation, each sub-ruler aligned with the distance ruler, each sub-ruler having tick marks disposed thereon to thereby create a plurality of measuring bars with the measuring bars of each sub-ruler corresponding to a characteristic carpet type having a given breadth; and a set of columns disposed between the distance ruler and the composite ruler, each column having a scaling equal to the distance ruler and composite ruler, each column identifying a characteristic carpet type and breadth, each column bearing a sequence of numbers thereon and with each number representing an areal dimension of a characteristic carpet type and breadth as cross-correlated to a measured distance as provided from the distance ruler.

Growing community of inventors

Midway, AR, United States of America

John W Guy