University of Minnesota

Other

The United States of America

John W Gronwald

David A Somers

Donald L Wyse

Burle G Gengenbach

Margaret A Egli

Sheila M Lutz

William B Parker

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells.

Methods and a maize acetyl CoA carboxylase gene for altering the oil content of plants

The present invention provides a complete cDNA sequence and partial DNA sequences encoding maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a maize acetyl CoA carboxylase gene in plant cells.

Transgenic plants expressing maize acetyl COA carboxylase gene and method of altering oil content

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and a method introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase crystallized enzyme.

Methods for expressing a maize acetyl CoA carboxylase gene in host cells

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.

Methods and an acetyl CoA carboxylase gene for conferring herbicide

The present invention provides an expression cassette encoding a plant acetyl CoA carboxylase gene and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. An expression cassette of the invention can comprise a gene coding a plant acetyl CoA carboxylase or a functional mutant thereof or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene combined with a promoter functional in a plant cell. The method of imparting herbicide tolerance to a plant includes the steps of introducing the expression cassette into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into a prokaryotic cell to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.

Method and an acetyl CoA carboxylase gene for conferring herbicide

Maize plants are grown in a planting area that are tolerant of inhibition by a cyclohexanedione herbicide, an aryloxyphenoxypropanoic acid herbicide, or mixtures thereof, at levels which normally inhibit growth and development, wherein said tolerance is conferred by an altered acetylcoenzyme A carboxylase (ACCase) tolerant of inhibition by the herbicide at levels which normally inhibit the activity of an unaltered ACCase. Additionally, an effective quantity of the named herbicide is applied so that the weeds (e.g., grass) are killed and the maize plants are uninjured. A representative cyclohexandione herbicide is sethoxydim and a representative aryloxyphenoxypropanoic acid herbicide is haloxyfop. Competition for the maize crop posed by weeds effectively is eliminated.

Method for combating weeds among maize plants

The present invention provides a maize plant wherein the growth and development of said plant is tolerant of inhibition by a cyclohexanedione herbicide, an aryloxyphenoxypropanoic acid herbicide, or mixtures thereof, at levels which normally inhibit the growth and development of maize, wherein said tolerance is conferred by an altered acetyl coenzyme A carboxylase (ACCase) tolerant of inhibition by said herbicide at levels which normally inhibit the activity of an unaltered ACCase.

Growing community of inventors

Shoreview, MN, United States of America

John W Gronwald