Darwin Discovery Limited

Qiagen Genomics, Inc.

Other

Operon Biotechnologies, Inc.

Blue Heron Biotechnology, Inc.

Ucb Pharma, S.a.

Oligoco, Inc.

Bonum Therapeutics, Inc.

University of Washington

Ucb S.a.

Celltech R&d Limited

Rapigene, Inc.

Gensinta, Inc.

Ucb Manufacturing, Inc.

John Thomas Mulligan

Jeffrey Van Ness

John C Tabone

Brian Robert Kovacevich

Mary E Brunkow

David J Galas

Bryan W Paeper

J Jeffry Howbert

David Guenther Winkler

Hsing-Yeh Parker

Gerard D Schellenberg

Ying-Hui Fu

Chang-En Yu

Junko Oshima

Shannon Lee Okada

Diane L Hollenbaugh

Justin Richard Killebrew

David J Winkler

Sharon R Long

Austen Heinz

Anselm Levskaya

Thomas T Egelhoff

R Gregg Brickner

Provided herein are protein complexes comprising a sensor domain and a therapeutic domain linked by a linker, and methods of use thereof. In aspects of the present disclosure, activity of the therapeutic domain comprises a dependence on sensor domain binding to target markers.

Compositions of protein complexes and methods of use thereof

A method of retrieving a subset of polynucleotide molecules from a mixture of polynucleotide molecules includes receiving a mixture of nucleotide sequences comprising one or more polynucleotide molecules, synthesizing one or more identifier (ID) regions onto the one or more polynucleotide molecules, and sequencing members of the population of polynucleotide molecules to associate the sequence of one or more of the molecules (the 'Polynucleotide Sequence') with the sequence of the attached ID region (the 'ID Sequence'). The method also includes generating a bead-bound library of one or more beads comprising subsets of identical polynucleotide molecules. Each bead is identified by the ID Sequence of the associated Polynucleotide Sequence. The method further includes sequencing the one or more ID regions of each bead to generate ID Sequence information for each bead, combining the Polynucleotide Sequence information, the one or more ID Sequences, and coordinates of each bead to identify the Polynucleotide Sequence on the bead, and retrieving the bead with its associated Polynucleotide Sequence from the flow cell based on the absolute coordinate position of the bead.

Method and apparatus for producing sequence verified DNA

A novel class or family of TGF-&#x3b2; binding proteins is disclosed. Also disclosed are assays for selecting molecules for increasing bone mineralization and methods for utilizing such molecules.

Compositions and methods for increasing bone mineralization

Described herein are pharmaceutical compositions comprising an anti-sclerostin antibody and an inhibitor of bone resorption.

Embodiments of the present invention include processing steps and subsystems, within automated-biopolymer-synthesis systems and within other automated systems for organic-chemistry-based processing, for removing reagent solutions and solvents from reaction chambers following various synthetic reaction steps and washing steps undertaken during biopolymer synthesis. Embodiments of the present invention employ any of various different types of liquid-absorbing materials to wick, or remove by capillary action, liquids from reaction chambers. Wicking-based methods and subcomponents of the present invention remove significantly greater fractions of solutions from reaction chambers than conventional methods and subsystems and, in addition, are mechanically simpler and produce fewer deleterious side effects than currently used methods and subsystems.

Wicking-based reagent-solution draining in an automated system

Embodiments of the present invention are directed to automated-polymer-synthesis systems that include discrete reagent-solution-addition, wait-time, and reagent-solution-draining sub-systems which together significantly increase throughput and decrease sub-system idle time. The automated-polymer-synthesis systems that represent embodiments of the present invention additionally include switches at points in which carriers can be received from multiple input paths or output to multiple different output paths. The automated-polymer-synthesis systems that represent embodiments of the present invention generally include an input spur and output spur in addition to a main loop, allowing carriers containing only completed polymers to be removed and new carriers input, so that carriers traverse the automated-polymer-synthesis systems independently from one another.

Automated polymer-synthesis system

Compositions and methods for increasing bone density using antibodies directed to a novel class or family of TGF-&#x3b2; binding proteins are provided. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases associated with a loss of bone density, for example osteoporosis.

Antibodies associated with alterations in bone density

Methods and compounds, including compositions therefrom, are provided for determining the sequence of nucleic acid molecules. The methods permit the determination of multiple nucleic acid sequences simultaneously. The compounds are used as tags to generate tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule. Each tag is correlative with a particular nucleotide and, in a preferred embodiment, is detectable by mass spectrometry. Following separation of the tagged fragments by sequential length, the tags are cleaved from the tagged fragments. In a preferred embodiment, the tags are detected by mass spectrometry and the sequence of the nucleic acid molecule is determined therefrom. The individual steps of the methods can be used in automated format, e.g., by the incorporation into systems.

Methods and compositions for determining the sequence of nucleic acid molecules

Nucleic acids relating to human TGF-&#x3b2; binding proteins are disclosed. Also disclosed are assays for selecting molecules for increasing bone mineralization and methods for utilizing such molecules.

Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.

Solid phase methods for polynucleotide production

The present invention discloses antibodies that specifically bind to a WRN gene product or a portion thereof.

Antibodies against gene products related to Werner's Syndrome

Growing community of inventors

Seattle, WA, United States of America

John Thomas Mulligan