Artel, Inc.

Seyonic S.a.

John Thomas Bradshaw

Richard H Curtis

Charles A Ewing

Ceara McNally

Keith J Albert

Alex L Rogers

Tanya R Knaide

Marc Boillat

George Rodrigues

David L Bohnsack

Kirby Pilcher

Alexis L Rogers

Ceara J Mcnally

Ceara Mcnally

A system and related method for improved liquid delivery evaluation using a solution containing one or more dyes such that the solution exhibits multiple distinct detectable light absorbance spectral features for calibrating or testing over extended volume or dilution ranges are described. The system includes: a photometric instrument capable of measuring optical absorbance at multiple wavelengths; one or more sample solutions to be dispensed using the liquid delivery system whose performance is being tested or calibrated; and vessels optionally pre-filled, or filled by the user, with diluent solution. The sample solutions contain one or more dyes, chosen so that multiple distinct detectable light absorbance spectral features, such as peaks and/or valleys and/or plateaus of the solution can be distinguished for volume or dilution ranges of interest. The concentrations of the dyes may be chosen so that a large volume delivery device is calibrated using a spectral feature in the solution with a low absorbance per unit pathlength, while a small volume delivery device is calibrated with the same sample solution but using a different spectral feature with a high absorbance per unit pathlength.

System and method for liquid delivery evaluation using solutions with multiple light absorbance spectral features

A liquid aspirating and dispensing apparatus and method of using the apparatus. Among other elements, the apparatus includes a liquid handling device having one or more channel members, each of which has a removable liquid holding tip attached, and a signal transmitting device. A signal transmitted from the signal transmitting device may be used in a variety of ways to yield information regarding the performance of the apparatus. This information may be provided via direct detection of the signal by an operator of the apparatus. Alternatively, the signal may be detected by a programmable signal detecting device, interpreted by the apparatus, and then displayed in a form that is understandable to the operator. Furthermore, the liquid holding tip may be modified to enable different forms of signal transmission.

Apparatus and method for aspirating and dispensing liquid

An apparatus used to calibrate microtiter plate readers. The apparatus includes one or more structures having two exterior surfaces wherein one of the exterior surfaces is coated with a coating selected to establish on that surface a reflection loss that mimics reflection loss at an air-liquid interface, and the other of the two exterior surfaces is coated with a coating selected to establish a reflection loss on that surface that mimics the reflection loss at a bottom surface of a microtiter plate. The apparatus may be a single layer, a multi-layered composition or a container. The apparatus is an artifact that may be used to calibrate a plate reader by mimicking a solution-filled microtiter plate. The artifact may be used for a plurality of liquids, including water. The artifact produces reflection losses more closely mirroring reflection losses expected for a liquid-filled microtiter plate well.

Artifact apparatus to mimic reflection losses of solution-filled microtiter plate readers and related uses thereof

A method for determining the volume of a test liquid aliquot used to calibrate a liquid delivery device. The method is useful for determining the volume of an aliquot of a test liquid which is complex, non-aqueous or both. A mixture contains the test liquid and a stock solution including a first dye, the first dye having absorbance characteristics which are measurably distinguishable from the absorbance characteristics of a second dye. The mixture is mixed with a diluent including the second dye to form a sample solution used to measure absorbances of the first dye and the second dye. The test liquid and the stock solution are combined gravimetrically in a controlled ratio so that the flow characteristics of the test-stock mixture correspond to the flow characteristics of the test liquid and without affecting the ability to distinguish the absorbance characteristics between the first dye and the second dye.

Calibrating dispensing device performance for complex and/or non-aqueous liquids

The invention provides ways to determine the impact of diluting a solution wherein the diluting may be carried out for any of a variety of purposes. In one embodiment, the method enables accurate volume dispensation calculations independent of meniscus shape. In another embodiment, the method enables accurate determination of plate washing efficiency. In yet another embodiment, the method enables the accurate determination of dilution ratio over a plurality of dilution steps. The methods described may be carried out using one or more systems arranged to perform the steps. A kit of the invention includes instructions for carrying out the steps of the methods and, optionally, one or more solutions suitable for conducting photometric measurements.

Quantitative dual-dye photometric method for determining dilution impact

An apparatus and related method for optical calibration of spectrophotometers is described. The apparatus is a calibration plate including one or more cuvettes filled with solutions of interest. The cuvettes are sealed to prevent evaporation. The cuvettes also possess a compressible component to allow for expansion of the solution and a bubble control apparatus to ensure that the compressible component does not intersect the beam path. A piece of neutral density glass is optionally included in the apparatus to track optical changes of the solutions over time.

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Gorham, ME, United States of America

John Thomas Bradshaw