Large Scale Proteomics Corporation

John Taylor, Jr

A computer-implemented image processing method and apparatus for combining a plurality of gel electrophoresis images. The method includes the steps of fitting the gel electrophoresis pixel intensity values for a subject pixel to a mathematical function, computing from the mathematical function a pixel intensity value according to a predetermined rule, and inserting the pixel intensity value into the composite image. The apparatus includes an image capturing device, and a computer having a memory and communicating with the image capturing device, the computer capable of receiving and storing into the memory a plurality of gel electrophoresis images from the image capturing device, the computer being further capable of fitting a plurality of intensity values of a pixel to a mathematical function over time and computing an optimal pixel intensity value for use in the composite image.

Gel electrophoresis image combining for improved dynamic range

A computer-implemented method analyzes a scanned image of a 2-D electrophoresis gel producing spot specific data (SSD). The computer creates an object pattern of a suitably processed scanned image and uses the spot information in the object pattern in order to warp a master pattern into alignment with the object pattern (and hence the scanned image). The object pattern is replaced with the warped master pattern, augmented by addition of well-defined spots in the object image not present in the warped master pattern, and optimized to fit a processed version of the scanned image. This new object model thus contains identifying and relative position information from the master pattern and other spot specific data (SSD) from the old object model. The new object pattern thereby forms a basis upon which to compare the scanned image with other scanned images similarly processed.

Method and apparatus for impressing a master pattern to a gel image

A computer-implemented image processing method and apparatus for warping a plurality of gel electrophoresis images is provided. The method includes the steps of assigning tiepoints in a reference image and in one or more object images. The tiepoints in the object image are evaluated one-by-one by comparison to regions about a corresponding tiepoint in the reference image, and the location of the tiepoint in the object image is adjusted by slight movement to a location with respect to recognizable features in both the reference and object image thereby defining a tiepoint pair linking a location in the reference image with a location in the object image. outlier tiepoint pairs may be rejected if that pair does not meet predetermined conditions. Warping functions are generated and then globally optimized. The plurality of images are tied together using the tiepoint pairs such that all of the images may be subsequently warped into registration to a single base image selected from the plurality of images.

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Clayton, NC, United States of America

John Taylor, Jr