Monsanto Technology LLC

University of Georgia Research Foundation, Inc.

John Tamulonis

David V Butruille

Vergel C Concibido

Kevin Lee Deppermann

Michael W Petersen

Sam R Eathington

Bruce J Schnicker

Heather M Forbes

James Narvel

Stanton B Dotson

George James Baley

John R LeDeaux

John W Pitkin

Chongqing Xie

Samuel R Eathington

Michael D Haverdink

Warren M Kruger

Jennifer L Yates

Kunsheng Wu

James Behm

David Vincent Butruille

John Robert Ledeaux

Nancy Sebern

Allen N Ondes

David W Finley

William M Fischer

John M Jensen

Roger L Lussenden

David R Wooten, Jr

Jennifer R Hicks

Charlotte A Sartell

Floyd G Hancock

Andrew D Nickell

Henry E Valentin

Jonathan Jenkinson

Richard Dougherty

Henry Roger Boerma

Terri B Hinchey

Kenneth Gruys

John F Phillips

Liesa Cerny

Bo-Keun Ha

Holly Kleiss

Qiang Zhang

Heather Forbes

A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

Methods of seed breeding using high throughput nondestructive seed sampling

The present invention relates to the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trail loci (QTL) for resistance theroot rot (PRR) caused by. The invention further includes the use of molecular markers in the introgression of PRR resistance QTL into soybean plants.

Methods and compositions for selecting soybean plants resistant to Phytophthora root rot

A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Automated systems for removing tissue samples from seeds, and related methods

The present invention is in the field of plant breeding and genetics, particularly as it pertains to the genus,. More specifically, the invention relates to a method for screening soybean plants containing one or more quantitative trait loci for disease resistance, species ofhaving such loci and methods for breeding for and screening ofwith such loci. The invention further relates to the use of exotic germplasm in a breeding program.

Method to identify disease resistant quantitative trait loci in soybean and compositions thereof

Methods and compositions for selecting soybean plants resistant to phytophthora root rot

The present invention is in the field of plant breeding. More specifically, the invention includes a method for breeding and selecting plants that uniform for one or more seed lot purity traits such as, such as distinct flower color, pubescence color, hilum color, and pod wall color. The invention further includes molecular markers associated with distinct flower color, pubescence color, hilum color, and pod wall color for uses in a breeding program.

Diagnostic molecular markers for seed lot purity traits in soybeans

Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes methods and compositions for breeding soybean plants containing quantitative trait loci that are associated with resistance to Brown Stem Rot (BSR), a fungal disease associated withspp. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring disease resistance for introgression into elite germplasm in a breeding program for resistance to BSR.

Methods and compositions for selecting soybean plants resistant to brown stem rot

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method of evaluating seeds for a desired trait, for example, for facilitating germplasm improvement activities, etc., generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, analyzing the tissue samples for one or more characteristics indicative of a desired trait, and selecting the sampled seeds that include the desired trait.

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Nevada, IA, United States of America

John Tamulonis