Bio-rad Laboratories, Inc.

New York Air Brake Corporation

John Sullivan

Waleed H Hassanein

Yan Wang

Tamer I Khayal

Richard Bringham

Xiao-Song Gong

Ahmed Elbetanony

Man Cheng

Ronald Taylor

Jeff Barnes

Ihab Abdel Fattah

Paul Murray

Greg Ritchie

Rongdian Fu

Evan H Bursey

Michael J Spadaccini

Kumar Deepak

Mark Anderson

Michael Van Driel

Tamer Khayal

Waleed Hassanein

Jeffrey Fisher

Robert Havener

Stanley Kyi

Robert Fishman

Tom Bishop

Anas Abdelazim

Scott Newell

Matthew De Remer

Doug Harriott

David Paul Levy

The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Methods and compositions for improving efficiency of nucleic acids amplification reactions

The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

The present invention provides DNA polymerases having increased efficiency of amplification of long amplicons. The present invention also provides for methods of amplifying target nucleic acid molecules with the DNA polymerases for increasing the efficiency of amplification of long amplicons.

Hybrid polymerases having the ability to produce long amplicons

The present interface system includes a controller monitoring pressure on a locomotive brake pipe port, controlling pressure on a train brake pipe port in response to the pressure on a locomotive brake pipe port, providing ECP commands on an ECP trainline via a train electrical terminal in response to the pressure on the locomotive brake pipe port, and providing electrical power on the ECP trainline via an train electrical trainline terminal from the locomotive electrical trainline terminal. The system includes a three position change over valve. The system also includes a wireless display unit which communicates via a transceiver with the controller and displays information from the controller to the operator remote from the interface system.

Change over valve for a transition system

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Oakland, CA, United States of America

John Sullivan