Lexent Bio, Inc.

John Spinosa

Kenneth Nesmith

Alexander Robertson

Haluk Tezcan

Nicole Lambert

Rohith Srivas

Timothy Wilson

Neil Peterman

Ram Yalamanchili

Peter George

The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may use a single flow cell to perform unbiased and/or biased sequencing to generate libraries of nucleic acid molecules. An aspect of the present disclosure provides a method for increasing complexity of a sample for sequencing, the method comprising: providing a first nucleic acid sample having a first degree of complexity that differs from a desired degree of complexity; providing a second nucleic acid sample having a second degree of complexity that differs from the first degree of complexity and that differs from the desired degree of complexity; pooling at least a portion of the first nucleic acid sample and at least a portion of the second nucleic acid sample, thereby generating a pooled nucleic acid sample having the desired degree of complexity; and sequencing at least a portion of the pooled nucleic acid sample.

Multiple sequencing using a single flow cell

Growing community of inventors

San Francisco, CA, United States of America

John Spinosa