Brookhaven Science Associates, Lcc

Dow Agrosciences LLC

Other

Cold Spring Harbor Laboratory

Assoicated Universties, Inc.

John Shanklin

Edgar Benjamin Cahoon

Tam Huu Nguyen

Edward J Whittle

Gunter Schneider

Terence Anthony Walsh

Ylva Lindgvist

Changcheng Xu

Jilian Fan

Chengshi Yan

Mark S Pidkowich

Ingo H Heilmann

Rob Martienssen

Alex Cantó-Pastor

Evan Ernst

Ylva Lindqvist

Xiao-Hong Yu

Yiheng Yan

Sanket P Anaokar

Richa Rawat Prakash

Almudena Molla-Morales

The invention provides mutant or variant oleosin polypeptides having one or more amino acid substitutions, particularly one or more arginine substitution for lysine, and having one or more amino acid deletions. The mutant oleosin polypeptides provide for higher triacylglycerol compared to wild type oleosin, including when the mutant oleosin is expressed in plants. Also provided are polynucleotides encoding the mutant oleosin(s), constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the mutant oleosin(s) and for producing oil in host cells and plants. The invention also relates to plants, particularly transgenic or recombinantly engineered plants, expressing one or more of the mutant oleosin polypeptides, as well as seeds and oil bodies derived from the plants.

Modified form of oleosin that when expressed in plants leads to increased triacylglycerol (oil) accumulation

Heterologous expression ofcyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression oftransferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), withcyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.

Engineering cyclopropane fatty acid accumulation in plants

Compositions and methods include genetically encoding and expressing a novel &#x394;-18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel &#x394;-18:0-ACP desaturase. In other embodiments, amino acid sequences have &#x394;-18:0-ACP desaturase activity. Methods can involve expression of &#x394;-18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

Accumulation of omega-7 fatty acids in plant seeds

The present disclosure provides methods and compositions for genetic transformation ofspecies.

Transformation of duckweed and uses thereof

The present invention discloses a novel gene encoding a transporter protein trigalactosyldiacylglycerol-5 (TGD5), mutations thereof and their use to enhance TAG production and retention in plant vegetative tissue.

Genes encoding novel lipid transporters and their use to increase oil production in vegetative tissues of plants

In the tgd1-1 mutant that displays substantially enhanced TAG synthesis and turnover, disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases FA turnover, leading to an increase in leaf TAG content up to 9% of dry weight and total leaf lipid by three-fold. The membrane lipid content and composition of tgd1-1 sdp1-4 and tgd1-1 pxa1-2 double mutants are altered and they are compromised in growth and development and fertility.

Enhanced triacylglycerol accumulation in vegetative tissues of plants

A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Combined hairpin-antisense compositions and methods for modulating expression

The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences forDesaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

ADS genes for reducing saturated fatty acid levels in seed oils

The present invention relates to methods for producing fatty acid desaturase mutants having a substantially increased activity towards substrates with fewer than 18 carbon atom chains relative to an unmutagenized precursor desaturase having an 18 carbon chain length specificity, the sequences encoding the desaturases and to the desaturases that are produced by the methods. The present invention further relates to a method for altering a function of a protein, including a fatty acid desaturase, through directed mutagenesis involving identifying candidate amino acid residues, producing a library of mutants of the protein by simultaneously randomizing all amino acid candidates, and selecting for mutants which exhibit the desired alteration of function. Candidate amino acids are identified by a combination of methods. Enzymatic, binding, structural and other functions of proteins can be altered by the method.

Mutant fatty acid desaturase and methods for directed mutagenesis

The present invention relates to oleate hydroxylase genes, proteins, and methods of their use. The present invention also relates to methods of using the oleate hydroxylase genes and proteins, including in their expression in transgenic organisms and in the production of hydroxylated fatty acids.

Isoform of castor oleate hydroxylase

The present invention relates to a method for producing mutants of a fatty acid desaturase having a substantially increased activity towards fatty acid substrates with chains containing fewer than 18 carbons relative to an unmutagenized precursor desaturase having an 18 carbon atom chain length substrate specificity. The method involves inducing one or more mutations in the nucleic acid sequence encoding the precursor desaturase, transforming the mutated sequence into an unsaturated fatty acid auxotroph cell such as MH13 , culturing the cells in the absence of supplemental unsaturated fatty acids, thereby selecting for recipient cells which have received and which express a mutant fatty acid desaturase with an elevated specificity for fatty acid substrates having chain lengths of less than 18 carbon atoms. A variety of mutants having 16 or fewer carbon atom chain length substrate specificities are produced by this method. Mutant desaturases produced by this method can be introduced via expression vectors into prokaryotic and eukaryotic cells and can also be used in the production of transgenic plants which may be used to produce specific fatty acid products.

Mutant fatty acid desaturase

Disclosed is a methods for modifying the chain length and double bond positional specificities of a soluble plant fatty acid desaturase. More specifically, the method involves modifying amino acid contact residues in the substrate binding channel of the soluble fatty acid desaturase which contact the fatty acid. Specifically disclosed is the modification of an acyl-ACP desaturase. Amino acid contact residues which lie within the substrate binding channel are identified, and subsequently replaced with different residues to effect the modification of activity.

Growing community of inventors

Shoreham, NY, United States of America

John Shanklin