New York University

John S Munger

Daniel B Rifkin

Justin P Annes

A method of screening for a protein involved in the extracellular regulation of latent TGF&#x3b2; activation by transducing a cell line with a retroviral cDNA library to create a reporter cell line that produces green fluorescent protein (GFP) in response to TGF-&#x3b2; signaling; growing individual clones created by the reporter cell line; co-culturing each individual clone with a second TGF-&#x3b2; reporter cell line that produces luciferase in response to TGF-&#x3b2;, wherein the luciferase production identifies positive clones; and identifying a mechanism of latent TGF-&#x3b2; activation that is employed by the positive clones. A TGF&#x3b2; reporter cell line including a cell line; and a retroviral cDNA library, wherein the reporter cell line produces GFP in response to TGF-&#x3b2; signaling. A method of screening for gene products involved in a biological process.

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New York, NY, United States of America

John S Munger