Columbia University

John Robert Edwards

Jingyue Ju

Zengmin Li

Yasuhiro Itagaki

Xiaoxu Li

Timothy H Bestor

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the &#x2014;OH group at the 3'-position of the deoxyribose.

Massive parallel method for decoding DNA and RNA

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the &#x2014;OH group at the 3'-position of the deoxyribose.

This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns.

Growing community of inventors

St. Louis, MO, United States of America

John Robert Edwards