General Electric Company

Ge Healthcare Bio-Sciences Corp.

Global Life Sciences Solutions Operations UK Ltd

Novartis Ag

Sommetrics, Inc.

Global Life Sciences Solutions USA LLC

Tokitae LLC

5I Sciences

Ce Healthcare Bio-Sciences Corp.

The University of Akron

Qiagen Healthcare Biotechnologies Systems Gmbh

John Richard Nelson

Robert Scott Duthie

Gregory Andrew Grossmann

Ryan Charles Heller

Christopher Michael Puleo

Anup Sood

Carl Williams Fuller

Erik Leeming Kvam

Patrick McCoy Spooner

Wei Gao

Shiv R Kumar

Ralf Lenigk

David Roger Moore

Anuradha Sekher

Nichole Lea Wood

Valentina Molteni

Matthew Jeremiah Misner

David C Tully

Jerome K Aarestad

Paul Vincent Rucker

Donatella Chianelli

Jason Thomas Roland

Donna Palmer

Richard Rose

Paul Michael Smigelski, Jr

Michael John Gerdes

Wei Cai

Su Lu

John Yupeng Gui

Craig Patrick Galligan

Cathryn Ellen Olsen

Li Zhu

Bahram Moasser

Zhixin Zheng

Erin Jean Finehout

Xiaodong Liu

Bing Li

Alison Myfanwy Wakefield

Gregory Andrew Grossman

Paulo Rangel

Paresh Lakhubhai Patel

Stephen James Capper

Sonali Jagdish Shah

Radislav Alexandrovich Potyrailo

Bernhard Hans Weigl

Peter James Tatnell

Alex David Corwin

Brian Michael Davis

Jeffrey Kenneth Horton

Brian Christopher Bales

David Andrew Shoudy

Todd Frederick Miller

William Christopher Alberts

Clifford Leslie Smith

William Patrick Waters

Christine Lynne Pitner

Zhida Pan

John J Macklin

Liangliang Qiang

Benjamin David Grant

John Thomas Connelly

Kwok Pong Chan

Arunkumar Natarajan

Mark McDougall

Andrew Soliz Torres

Victoria Eugenia Cotero

Liladhar Murlidhar Waykole

Michael James Rishel

Steven Y Go

Andrew Arthur Paul Burns

Abraham Joy

Lakshmi Sireesha Kaanumalle

Vicki Herzl Watkins

Nandini Nagraj

Anthony John Murray

Feng Chen

Xiaoyang Wang

Timothy James Yosenick

John E Macklin

Xiaohui Chen

Suzana Kiel

Christine Lynne Surrette

Wei Sun

Jason Michael Nichols

Paul Mitsis

Andrew David Pris

Haiguang Xiao

Yubo Zhang

Alexander Schenk

Lisa Anne Lowery

R Scott Duthie

Runyan Li

Haiying Wang

Sudhakar Rao

Chockalingam Palaniappan

Preetha Biswas

Mark A Mozola

Lisa Pinkava

Nicholas Nun

The subject matter of the present disclosure generally relates to techniques for addressing or correcting dysregulation of the trauma regulation pathway. The dysregulation may be associated with a physiological condition, such as a SARS-CoV-2 viral infection. In an embodiment, the techniques include treating dysregulation based on a renin-angiotensin pathway molecule or cell and/or a splenic pathway molecule or cell using targeted neuromodulation. In an embodiment, neuromodulation is used to regulate the immune system, e.g., as an energy-based adjuvant for a vaccine.

Dysregulation of trauma regulation pathway treatment and monitoring techniques

A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.

Method for nucleic acid analysis directly from an unpurified biological sample

A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5' flanking sequence and a donor 3&#x2032; flanking sequence. The donor 5&#x2032; flanking sequence and the donor 3&#x2032; flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.

Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences

A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

Device for rapid detection of tuberculosis-lipoarabinomannan (TB-LAM) with enhanced sensitivity

A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2&#xd7; to 25&#xd7; compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

Method and associated device for rapid detection of target biomolecules with enhanced sensitivity

An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.

Electrospun fibers for protein stabilization and storage

Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

Cell-free protein expression using rolling circle amplification product

Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.

Endonuclase-assisted isothermal amplification using contamination-free reagents

Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.

Method and device for collection and amplification of circulating nucleic acids

A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

Methods for electroelution of biomolecules

The present invention relates to compounds of Formula I, a stereoisomer, enantiomer, a pharmaceutically acceptable salt or an amino acid conjugate thereof; wherein variables are as defined herein; and their pharmaceutical compositions, which are useful as modulators of the activity of Farnesoid X receptors (FXR).

Compositions and methods for modulating farnesoid X receptors

Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/&#x3bc;L to about 35 ng/&#x3bc;L. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.

Cell-free protein expression using double-stranded concatameric DNA

A method is provided herein, wherein the method of capturing a target nucleic acid, comprises applying a nucleic acid capture probe to a capture zone of a needs definition, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate. The method further comprises applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate; wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization to the capture zone.

Methods for capturing nucleic acids

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Clifton Park, NY, United States of America

John Richard Nelson