Biolegend, Inc.

Axiom Biotechnologies, Inc.

Saryna Biotechnologies LLC

Vivreon Biosciences, LLC

John Ransom

Dhanesh Gohel

Hong Zhang

Alex Okun

Ilya Okun

Teresa A Bennett

Andrew Newman

Milton L Greenberg

Clayton A White

Omed S Muzaffery

Andrew Beernink

David J Sieg

Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.

Stable nanomagnetic particle dispersions

The methods of the invention employ targeted magnetic particles, preferably targeted nanomagnetic particles, and targeted buoyant particles such as buoyant microparticles and microbubbles. Among the benefits of the invention is the ability to combine targeted magnetic particles with differentially targeted buoyant particles to achieve separation of two or more specifically cell targeted populations during the same work flow.

Compositions and methods for performing magnetibuoyant separations

Described herein are compounds that modulate the activity of CRAC channels, methods of making such compounds, pharmaceutical compositions, and medicaments containing such compounds, and methods of using such compounds in the treatment of conditions, diseases, or disorders associated with CRAC channel activity.

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A system and method of mixing and injecting discrete sample mixtures into a flow cytometer or other sample analysis apparatus may generally comprise a sample injection guide coupling a liquid handling apparatus with a sample analysis apparatus and facilitating injection of discrete sample mixtures into a fluidic system of the apparatus.

Direct mixing and injection for high throughput fluidic systems

An apparatus and method for real-time measurement of a cellular response of a test compound or series of test compounds (,) on a flowing suspension of cells (,), in which a homogeneous suspension of each member of a series of cell types (,) is combined with a concentration of a test compound (,), directed through a detection zone (,), and a cellular response of the living cells is measured in real time as the cells in the test mixture are flowing through the detection zone (,). The apparatus may be used in automated screening of libraries of compounds, and is capable of real-time variation of concentrations of test and standard compounds and generation of dose/response profiles within a short time span.

Cell flow apparatus and method for real-time of cellular responses

Cell flow apparatus and method for real-time measurements of cellular responses

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Encinitas, CA, United States of America

John Ransom