Sloan Valve Company

Wilson Wolf Manufacturing Corporation

Other

Baylor College of Medicine

The Government of the United States of America As Represented by the Secretary of the Deptartment of Health and Human Services

St. Jude Medical, Inc.

Wheaton Industries, Inc.

Numedis Incorporated

501 Sloan Valve Company

Sloan Valley Company

Allovir, Inc.

Arichell Technologies Inc.

John R Wilson

Daniel P Welch

Douglas A Page

Alison Robeck

Cliona M Rooney

Ann Marie Leen

Juan F Vera

William Wilson

Charles S Allen

Joseph J Pilolla

Kay Herbert

Dan Welch

Xiaoxiong Mo

Timothy Ames

Martin L Wolf

Joshua D Anthony

Steven R Oliver

Klaus Renner

Joshua P Kinsley

Peter J Jahrling

Xu Wang

Alfred J Costa

Matthew T Kowalczyk

Natan E Parsons

Fatih Guler

Martin E Marcichow

David J Carpenter

Jerry P Gronwick

Ulrike Gerdemann

John Beckman

Steven C Meier

James Kendall

Dana K Griffin

Harald Quintus-Bosz

Matthew Thomas Kowalczyk

Klaus H Renner

Steven A Rosenberg

Matthew W Weston

Debra L Skelnik

Nhon T Vuong

M William Mirsch, Iii

Wei C Jin

Robert L Somerville

Roger D Wilson

Juan Fernando Vera Valdes

Qiaohong Chen

Jonathan W Snyder

Rainer Ludwig Knaus

Mark E Dudley

Daniel J Carroll

Jean Daher

James Counaghton Allen

Katy Rebecca Newton

Richard A Kamysz

Marianna Sabatino

Jon Demczewski

Edward M Jones

Peter Golen

Jianjian Jin

Conrad L Jahrling

Kathy J Grossinger, Legal Representative

Robert L Meisch

Beverley I Lundell

David Stroncek

Efstathios S Avgoustiniatos

Klearchos K Papas

Kay Ph Herbert

Jean F Daher

James Connaughton Allen

Wei Jin

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

Highly efficient gas permeable devices and methods for culturing cells

An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cmif the growth surface is not comprised of gas permeable material and up to 2 ml/cmif the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×10cells/cmat the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×10cells/cm.

Methods of cell culture for adoptive cell therapy

An in vitro expansion process for rapid expansion of antigen specific T cells, such as allogeneic antigen specific T cells comprising the steps culturing in a gas permeable vessel a population of PBMCs (such as allogeneic PBMCs) in the presence of antigen, for example a peptide or peptide mix relevant to a target antigen(s), in the presence of an exogenous cytokine characterized in that the expansion to provide the desired population of T cells is 14 days or less, for example 9, 10, 11 or 12 days, such as 10 days. The disclosure also extends to T cell populations generated by and obtained from the method and the use of same in therapy.

Process for T cell expansion

Improved cell culture devices and related methods that overcome the limitations of prior devices and methods, by creating devices that can integrate a variety of novel attributes. These various attributes include the use of gas permeable material and medium volumes that exceed conventional devices as well as compartments that can facilitate the long term study of high density cultures with reduced disruption of the culture environment, the ability to study the migration of items of interest including substances such as chemokine, track the movement of cells, and monitor cell to cell interactions.

Culture methods and devices for testing

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

Closed system device and methods for gas permeable cell culture process

An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cmif the growth surface is not comprised of gas permeable material and up to 2 ml/cmif the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5&#xd7;10cells/cmat the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25&#xd7;10cells/cm.

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

Cell culture methods and devices utilizing gas permeable materials

An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.

Methods of growing tumor infiltrating lymphocytes in gas-permeable containers

Water supply adapter

A dunnage bag and load strap hanger apparatus includes a dunnage bag and load strap hanger and a handle mounted applicator block operative to support and affix the dunnage bag and load strap hanger. The dunnage bag and load strap hanger is fabricated from three distinct and separate parts: a generally planar support substrate and two release papers covering adhesive patches or regions on the support substrate. The support substrate in one embodiment is a sheet that is further divided into two contact members separated by a resilient coupler. Each contact member preferably has a contact body, a hanging loop distal to the resilient coupler, and in alternative embodiments an adhesive coating or other fasteners and affixers. In one alternative embodiment there is one affixing surface, and a fastener to couple two contact members together to support a load strap.

Dunnage bag and load strap hanger apparatus and method

A dunnage bag and load strap hanger apparatus includes a dunnage bag and load strap hanger and a handle mounted applicator block operative to support and affix the dunnage bag and load strap hanger. The hanger is fabricated from three distinct and separate parts: a generally planar support substrate, adhesive patches or regions on the support substrate, and release paper covering the adhesive patches. The support substrate in some embodiments is a sheet that is further divided into two contact members separated by a resilient coupler. Each contact member preferably has a contact body, a hanging loop distal to the resilient coupler, and in alternative embodiments an adhesive coating or other fasteners and affixers. In one embodiment there is one affixing surface, and a fastener to couple two contact members together to support a load strap. In another alternative embodiment a load strap is woven through a pair of slots.

Growing community of inventors

Naperville, IL, United States of America

John R Wilson