Micronics, Inc.

Perkinelmer Health Sciences, Inc.

John R Williford

C Frederick Battrell

John Clemmens

Denise Maxine Hoekstra

Isaac Sprague

Jason Capodanno

John Gerdes

Joan Haab

Carolina L Elmufdi

Diane Wierzbicki

Stephen Mordue

Mark Stamatios Kokoris

Melud Nabavi

Wayne L Breidford

Elizabeth Reeves Leber

Sabrina N Gunderson

Sabrina N Gates

Elizabeth R Leber

Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

Microfluidic apparatus and methods for performing blood typing and crossmatching

Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices

Formulations for dry storage of PCR reagents are described. These formulations find use in manufacture of self-contained microfluidic card devices for PCR clinical testing in which the reagents are reconstituted at the point of testing. In these cards, TAQ polymerase is stored 'on-board' in vitrified dry form without lyophilization or freezing, and is reconstituted by either the sample or a sample eluate during the assay.

Rehydratable matrices for dry storage of TAQ polymerase in a microfluidic device

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.

Integrated nucleic acid assays

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.

Methods and devices for microfluidic point-of-care immunoassays

Growing community of inventors

Sammamish, WA, United States of America

John R Williford