Igen International, Inc.

Igen, Inc.

John R Link

John H Kenten

Satyanarayana R Gudibande

A process for exponentially amplifying a selected nucleic acid sequence present in a sample, comprising the steps of forming a mixture of the sample and a single primer designed to hybridize with the selected nucleic acid sequence; causing the single primer to hybridize to a single strand of the nucleic acid sequence of interest; forming a duplex product of the nucleic acid by a polymerase reaction; separating the duplex product into single strands; and repeating the preceding steps until the rate of production of the amplification product is exponential and the nucleic acid sequence of interest has been amplified.

Method for making a primer and nucleic acid exponential amplification methods using said primer

A method of detecting a nucleic acid sequence of interest in the amplification product of a polymerase chain reaction or other primer directed reaction comprising the steps of:

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Springfield, VA, United States of America

John R Link