Cytonome/st, LLC

Cytonome, Inc.

Asia Pacific Medical Technology Development Company, Ltd

Wispry, Inc.

Other

Harvard College

John R Gilbert

David A Weitz

Manish Deshpande

Sebastian Böhm

Bernard Bunner

Hugh Lewis

Jaishree Trikha

Edward L Sinofsky

Andrew Johnson

Derek Beaupre

Arthur Sherman Morris, Iii

Dana Richard DeReus

Shawn Jay Cunningham

Yih-Sharng Chen

Subham Sett

Siebe Bouwstra

Randy J Richards

Francois-Xavier Musalem

Chih-Hsien Wang

John Heyman

Nghia Hung Chiem

John Harley

Richard Day

Sebastian B&ouml;hm

Renata Patton

Manish Desphande

Ed Sinofsky

Arthur S Iii Morris

J Richards Randy

A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

Multilayer hydrodynamic sheath flow structure

The present disclosure describes for at least two zones of selective thermal therapy of the body. Three-port extracorporeal circuits are described that can be used to establish at least two zones of selective thermal therapy of the body. The example three-port extracorporeal circuit includes a branching section that provides for setting the temperature of blood injected into two different portions of the body at differing temperature levels, to provide. at least two zones of selective thermal therapy.

Systems and methods for providing zones of selective thermal therapy

The present disclosure describes flow stagnation control components that allow improved flow control in systems including injection members, while also limiting the creation of regions of little to no flow in the vasculature, resulting in low flow zones or dead zones. The flow stagnation control components can be formed as an imposed minimum conductance component or a controlled flow partitioning system.

Systems and methods for flow stagnation control

The present disclosure describes effector deployment systems and devices that can be coupled adjunctively to a shaft of a concentric cylinder system, to provide additional functionality during intravascular procedures.

Adjunctive localization systems and devices

A microfluidic device includes a microchannel having an interior bounded by a side wall, an inlet, a switching region, and a plurality of outlet channels downstream of the switching region. The microchannel is formed in a microfluidic chip substrate and configured to accommodate a flow of liquid through the microchannel. The microfluidic device includes a valve operatively coupled to the switching region comprising a sealed reservoir. A side passage extends between the reservoir and the interior of the microchannel via an aperture in the side wall and is configured to accommodate a volume of liquid between the interior of the microchannel and the reservoir. The microfluidic device includes an actuator integrated into the microfluidic chip and configured to increase an internal pressure of the reservoir and move at least a portion of the volume of the liquid from the side passage into the microchannel to deflect a portion of the liquid flowing through the microchannel.

Methods for controlling fluid flow in a microfluidic system

An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.

Optical detector for a particle sorting system

An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.

Actuation of parallel microfluidic arrays

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

Unitary cartridge for particle processing

The example systems, apparatus and methods use a local perfusion extracorporeal circuit (LPEC) for perfusing a local target region of a body, with a systemic perfusion extracorporeal circuit (SPEC) coupled to the core region of the vasculature using a peripheral placed loop to the body, and a control procedure to cause the local target region of the body to be at a specified pattern of temperature values that are different than the temperature of the core of the body.

Systems and methods for extracorporeal support

A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises actuators and chambers for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.

Method and apparatus for sorting particles

Microfluidic device

Growing community of inventors

Brookline, MA, United States of America

John R Gilbert