Provalis Diagnostics Ltd

Quotient Diagnostics Limited

Ekf—diagnostic Gmbh

John Phillip Vessey

David Alan Percival

John Worthington Attridge

David Andrewes

John Curtis

Anthony Stevenson

Adrian Richard Gray

Mark Cresswell

Anthony O'Dell

David Percival

John Anthony Odell

David Griffiths

Mark Gresswell

A method and apparatus to estimate the concentration of a non-fluorescent substance (e.g. haemoglobin) in a fluorescent assay by separately estimating the non time dependent alteration attributed to inherent filter effects from the time dependent alteration caused by the assay chemistry. Such a method obviates the requirement for a separate photometric or other measurement thereby simplifying the methodology and associated instrumentation.

Fluorescent assay

A method and apparatus to estimate the concentration of a target substance (e.g. Cholesterol or CRP) in the plasma component of awhole blood sample without the need to separate the red blood cells from the plasma prior to testing, thereby simplfying the design and construction of the test device. The invention achieves this by measuring the analyte under investigation in a time dependent (bio-/immuno-) chemical reaction and measuring separately, a marker substance (e.g haemoglobin) for the estimation of red blood cell volume, using a non-time-dependent alteration in physical property of the reaction mixture (in this instance, transmission) attributed to inherent filter effects on sample addition. These non-time-dependent changes are not part of the reaction chemistry, and are resolved from the time dependent alteration in physical property caused by the assay chemistry by continuous measurement and mathematical modelling. Algorithms that combine these two parameters are used to estimate the target substance and compensate for variations in the percentage haematocrit of the sample. The method equalises the assay response for subtle variations in patient sample (e.g. haematocrit).

Whole blood assay

A apparatus for assaying glycated proteins and other analytes in biological samples such as blood, in which a sample is presented to the apparatus, includes an inlet port between moveable between first and second inlets, such that the inlet can be brought into liquid communication with each inlet in turn. The inlet port accommodates a filter or binder. The apparatus also includes a microprocessor operable via a key pad, at least one light emitter and at least one light detector, a display and driver, and an A to D converter, and is operatively connected to a power source.

Device and apparatus for conducting an assay

Spectrophotometer

Sample holder for spectrophotometer

Growing community of inventors

East Horsley, United Kingdom

John Phillip Vessey