Novo Nordisk A/s

Unizyme Laboratories A/s

John Pedersen

Henrik Dalbo Slashedge

Thorkild Christensen

Jo Slashedrli Ringsted

Torben E Jessen

Leif Kongerslev

Conni Lauritzen

Mads Thorup Madsen

Enzymatic process for producing a desired protein from an amino terminal

Process for preparing a desired protein

Amino-terminally extended human growth hormone (HGH)

Biosynthetic human growth hormone

A process for producing ripe human growth hormone is described which comprises introducing into bacteria a recombinant DNA vector comprising a DNA sequence encoding an amino-terminal extended human growth hormone which amino-terminal extension has a negatively charged amino acid sequence and has an even number of amino acids, growing the bacteria such that the amino-terminal extended human growth is expressed, separating the amino-terminal extended human grown hormone from contaminants, cleaving the amino-terminal extension with dipeptidyl aminopeptidase I to obtain ripe human growth hormone and isolating the ripe human growth hormone produced.

In a method for effecting an increased expression in serum-free medium of recombinant proteins in a host cell being able to express said protein said host is cultured in a serum-free medium comprising an egg yolk fraction being free of lipoprotein and lipids so as to express said protein. Also the use of an egg yolk fraction being free of lipoprotein and lipids for increasing the expression of recombinant proteins in a host cell being able to express said protein in a serum-free cell growth medium is disclosed.

Recombinant protein production in serum-free medium

Growing community of inventors

Kokkedal, Denmark

John Pedersen