Children's Medical Center Corporation

Pendar Technologies, LLC

John Padraic Romfh

Daryoosh Vakhshoori

Peili Chen

Brian David Polizzotti

John N Kheir

Lois Elaine Smith

Bertan D Cakir

Joshua Salvin

Alison Perry

Apparatuses and methods for using Raman Resonance Spectroscopy to evaluate metabolic and oxygenation status of the eye are disclosed herein. In some embodiments, metabolic mapping of the eye may be performed by aligning a Raman spectrum and a recorded spatial image of the eye.

Resonance Raman spectroscopy for evaluating mitochondrial function

The inventors have developed tools for quantifying the mitochondrial redox state of in vivo, in situ tissue using resonance Raman spectroscopy. The tissue is illuminated with an excitation beam that causes the tissue to scatter Raman-shifted light, which is collected and analyzed to produce coefficients representing the relative concentrations of different chromophores in the tissue. These relative concentrations indicate the redox state of whole mitochondria, hemoglobin oxygen saturation, myoglobin oxygen saturation, and/or redox state of individual cytochrome complexes in mitochondria of the in vivo, in situ tissue. Quantifiable information about these states and/or saturations can be used to assess tissue health, including organ (dys)function before, during, and after surgery. For example, this information can be used to predict impending cardiac failure, to guide surgical interventions, to monitor organ health after transplantation, or to guide post-operative care.

In-vivo monitoring of cellular energetics with Raman spectroscopy

Growing community of inventors

Cambridge, MA, United States of America

John Padraic Romfh