Monsanto Technology LLC

Monsanto Company

John P Purcell

John T Greenplate

David Richard Corbin

Michael Spencer

Brenda A Lowe

Jyoti R Rout

Robert D Sammons

Melissa Way

Michael G Jennings

Anne Spelletich

Charles P Romano

Jay C Pershing

Barbara Isaac

The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

Methods for rapidly transforming monocots

Compositions and methods for controlling insects by co-expressing an amino acid oxidase and a second enzyme that provides insecticidal activity when present in a mixture with the amino acid oxidase are disclosed. Also disclosed are DNA and protein sequences, and transformed microorganisms and plants useful for achieving such insect control.

Methods and compositions for controlling insects

3-Hydroxysteroid oxidase in combination with Bacillus thuringiensis CryIA(b) or CryIA(c) crystal proteins or polypeptides controls insects, particularly lepidopterans. The combination when applied to larvae of several lepidopterans indicated significant effects on mortality and stunting and indicated responses that were statistically significant over those expected from additive, independent action. Cholesterol oxidase and CryIA(b) crystal protein were also effective against black cutworm neonates when co-expressed in transgenic corn and tested in a detached leaf assay.

Method of controlling insects

3-Hydroxysteroid oxidase controls insects, particularly lepidopteran and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms, thereby providing a method of controlling insect infestation.

3-Hydroxysteroid oxidase controls insects, particularly lepidopterans and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of controlling insect infestation.

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Ballwin, MO, United States of America

John P Purcell