Miles Laboratories, Inc.

Miles Inc.

John P Chiang

Oreste J Lantero

Bhalchandra H Patwardhan

David J Solow

Timothy A Bemish

A water-immiscible organic extractant system can economically be used to recover citric acid from molasses fermenter beer containing a high salt content by using a process combination of passing the aqueous raffinate from the citric acid extraction step through a basic anion-exchange resin to adsorb residual citric acid and release citrate anions from previously formed salt cation-citrate compounds. Trisodium citrate is then eluted from the resin by passing aqueous sodium hydroxide therethrough.

Production of citric acid and trisodium citrate from molasses

Disclosed is a process whereby enzymes are immobilized on granular diatomaceous earth. The process involves treating the diatomaceous earth with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the diatomaceous earth leaving pendant amine groups free to react further. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of an enzyme or enzymes can be covalently bound to the polyamine via the amine reactive compound.

Immobilization of biocatalysts on granular diatomaceous earth

Disclosed is a method for preparing transglucosidase comprising culturing a strain of Talaromyces duponti in a suitable nutrient growth medium and isolating the transglucosidase therefrom. Also disclosed is the transglucosidase produced by T. duponti.

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Elkhart, IN, United States of America

John P Chiang