United States of America, As Represented by the Secretary of Agriculture

Institut Pasteur

University of Minnesota

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Iowa State University Research Foundation, Inc.

Oregon State University

Institut National De La Recherche Agronomique (inra)

The United States of America As Represented by the Department of Agriculture/cooperative State Research Education and Extension Service (usda/csrees)

John P Bannantine

Sylvie Bay

Gilles Etienne

Franck Biet

Vivek Kapur

Ling-Ling Li

Qing Zhang

Alongkorn Amonsin

Marc D Porter

Neil P Price

Robert J Lipert

Michael A Jackson

Judith R Stabel

Betsy Jean Yakes

Daniel D Rockey

Trina M Hartman

The present invention is directed to an isolated synthetic tripeptide of formula H-D-Phe-N-Methyl-L-Val-L-Ala-OMe (SEQ ID NO: 1), or a derivative thereof, and to the corresponding lipotripeptides, which are specific tosubsp.(Map) S-type strain, as well as derivatives and conjugates thereof. The invention also concerns the use of these antigens in different methods and tests for detecting Map infection, especially by detecting humoral response and cell mediated response of infected animals. The invention is also directed to a genetic signature of Map and a mass spectrometry and NMR spectroscopy signature of Map presence or infection.

Antigenic tripeptides derived fromsubsp.S-type strains, derivatives and uses thereof

The invention relates to new tunicamycin structures comprising N-acyl groups metabolically integrated into the terminus of the TUN via thebranched chain fatty acid pathways and variants thereof, and methods of preparing such new tunicamycin structures. The invention further relates to antibacterial compositions comprising such new tunicamycin structures, and methods for using such antibacterial compositions for killing Gram-positive bacteria.

Omega-alicyclic tunicamycins and analogs

The present invention is directed to an isolated synthetic tripeptide of formula H-D-Phe-N-Methyl-L-Val-L-Ala-OMe (SEQ ID NO:1), or a derivative thereof, and to the corresponding lipotripeptides, which are specific tosubsp.(Map) S-type strain, as well as derivatives and conjugates thereof. The invention also concerns the use of these antigens in different methods and tests for detecting Map infection, especially by detecting humoral response and cell mediated response of infected animals. The invention is also directed to a genetic signature of Map and a mass spectrometry and NMR spectroscopy signature of Map presence or infection.

Antigenic tripeptides derived fromsubsp.s-type strains, derivatives and uses thereof

Compositions of immunogenic proteins ofsubspecies(MAP) are effective for stimulating a protective immune response in animals against MAP. Combinations of MAP1087 with two or more of MAP1204, MAP1272c and MAP2077c are effective for stimulating a protective immune response in animals against MAP and can be administered as vaccines against(Johne's disease). Induction of the immune response significantly reduces or eliminates colonization of the animal by MAP, and consequently reduces or eliminates the symptoms of clinical disease in animals infected with MAP and reduces or eliminates fecal shedding of MAP. Vaccination with the compositions provides protection against clinical disease and reduces transmission of MAP infection within a herd.

Recombinant mycobacterium avium subsp. paratuberculosis proteins induce immunity and protect against infection

Hybridoma cell lines which produce and secrete monoclonal antibodies which selectively bind tosubspecieshave been produced. Cells ofsubspeciesin biological samples may be detected and quantified by contacting the sample with the antibodies to form asubspecies/antibody immunocomplex whensubspeciesis present, which immunocomplex may then be detected. The monoclonal antibodies also may be incorporated into kits for the detection and quantification ofsubspecies

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Hybridomas producing highly specific monoclonal antibodies to detect mycobacterium avium subspecies paratuberculosis

The present invention provides nucleic acid molecules unique to. The invention also provides the polypeptides encoded by the-specific nucleic acid molecules of the invention, and antibodies having specific binding affinity for the polypeptides encoded by the-specific nucleic acid molecules. The invention further provides for methods of detectingin a sample using nucleic acid molecules, polypeptides, and antibodies of the invention. The invention additionally provides methods of preventing ainfection in an animal.

Mycobacterial diagnostics

A process of detection of the causative agent of Johne's disease (subsp.) (MAP) by detecting shedding of surface protein of MAP. A preferred way is use of surface enhanced Raman Spectroscopy. The system of detecting MAP shedding of protein provides early detection and diagnosis, and therefore allows early treatment for Johne's disease in ruminant animals.

Bioamplification for microbial sensor signal transduction

The present invention provides nucleic acid molecules unique toThe invention also provides the polypeptides encoded by the-specific nucleic acid molecules of the invention, and antibodies having specific binding affinity for the polypeptides encoded by the-specific nucleic acid molecules. The invention further provides for methods of detectingin a sample using nucleic acid molecules, polypeptides, and antibodies of the invention. The invention additionally provides methods of preventing ainfection in an animal.

Certain Chlamydia proteins have been found to be infection-specific and to be associated primarily with the vegetative Reticulate Body form of Chlamydia rather than with the refractile Elementary Body form of Chlamydia. The invention includes a vaccine directed against the Reticulate Body form of Chlamydia comprising one or more infection-specific proteins, or fraction thereof; a method of using such a vaccine; a method of production of such a vaccine; a method for detection of infection-specific antibodies in a biological specimen; a method for detection of infection-specific antigens in a biological specimen and a method of using therapeutic agents specifically directed against infection-specific peptides, or the genes that code for such peptides, to treat chlamydial infection. The invention also includes the IncB, and IncC proteins of , and nucleotides encoding these proteins, ant the TroA, TroB and p242 proteins of , and the nucleotides that encode polypeptides.

Growing community of inventors

Ames, IA, United States of America

John P Bannantine