Roche Molecular Systems, Inc.

John Niemiec

Thomas W Myers

David H Gelfand

Keith Bauer

Stephen Gordon Will

Amar Gupta

Nancy J Schoenbrunner

Walter J Laird

Veeraiah Bodepudi

Dirk Zimmermann

Nicolas Newton

Edward Soh Smith

Meike Eickhoff

Christopher D Newhouse

Niclas Hitziger

Heike Wilts

Anke Mussmann

The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification.

Generic PCR

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2'-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2&#x2032;-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

2'-terminator related pyrophosphorolysis activated polymerization

The present invention provides modified primers for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified primers result in less template-independent non-specific product (primer dimer) compared to amplifications carried out using unmodified primers.

Amplification using modified primers

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San Leandro, CA, United States of America

John Niemiec