Biomerieux Sa

Biomerieux, Inc.

John Mills

Sylvain Orenga

David Mosticone

Antoine Vimont

Marie Cellier

The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of thegroup comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.

[object Object]

The present invention relates to a method for identifying bacteria of thegroup, comprising the following steps: (a) providing a sample that may contain bacteria of thegroup, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of thegroup by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of thegroup is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to thegroup, that may be present in the sample.

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Fenton, MO, United States of America

John Mills