National Technology & Engineering Solutions of Sandia, LLC

University of California

Battelle Memorial Institute

Sandia Corporation

Pacific Northwest National Laboratory

Natl Tech & Engineering Solutions of Sandia, LLC

John Michael Gladden

Blake A Simmons

Harsha D Magurudeniya

Steven Singer

Jon K Magnuson

Scott E Baker

Lalitendu Das

Ezinne Achinivu

Hemant Choudhary

Ryan Wesley Davis

James Kirby

Benjamin Chiau-Pin Wu

Joonhoon Kim

Joshua I Park

Venkataramana R Pidatala

Weihua Wu

Kaixuan Huang

Morgann C Reilly

Jed J Lynn

Ken Sale

Di Liu

Gina Marie Geiselman

Mood Mohan

Jay D Keasling

Seema Singh

Taek Soon Lee

Tanmoy Dutta

Jian Sun

Samuel Coradetti

Jennifer Hiras

Sebastian D Kolinko

Evelyn Denzel

Alberto Rodriguez

Nawa Raj Baral

Jinxiang Zhang

Junko Yaegashi

HeeJin Hwang

Peter Britton Otoupal

Saori Amaike Campen

Jeffrey M Skerker

The present invention provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent comprising a polyamine, or a mixture of polyamines, to a biomass to dissolve at least part of solid biomass in the solvent, wherein the polyamine is a Brønsted or Lewis base, and/or the polyamine is a hydrogen bond donor and/or acceptor; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; (c) optionally separating the sugar from the solubilized biomass mixture; and (d) optionally separating the lignan from the solubilized biomass mixture.

Use of polyamines in the pretreatment of biomass

The present invention provides for a method to deconstruct a biomass comprising: (a) introducing one or more individual components of an ionic liquid (IL) or deep eutectic solvent (DES) to a biomass, wherein the one or more individual components, and optionally any components already present in the biomass, form an IL or DES, or mixture thereof, which solubilizes the biomass to form a solubilized biomass mixture, wherein at least one individual component is introduced to the biomass separately from any other individual component; (b) optionally introducing an enzyme and/or a microbe to the solubilized biomass mixture such that the enzyme and/or microbe produces a sugar from the solubilized biomass mixture; and, (c) optionally separating the sugar from the solubilized biomass mixture.

Use of in-situ ionic liquid (IL) and deep eutectic solvent (DES) synthesis using chemically synthesized or biomass-derived ions in the pretreatment of biomass

The present invention provides for a method to deconstruct a biomass: the method comprising: (a) ensiling a biomass to produce comprising one or more organic acids, and (b) introducing a solvent to the ensiled biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent is an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture.

Use of ensiled biomass for increased efficiency of the pretreatment of biomass

The present invention also provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent to a biomass to dissolve at least part of solid biomass in the solvent, wherein the solvent comprises (i) a metal salt, and (ii) an ionic liquid (IL) or deep eutectic solvent (DES), or mixture thereof, to form a solubilized biomass mixture; (b) optionally introducing an enzyme and/or a microbe to the composition such that the enzyme and/or microbe produce a biofuel and/or chemical compound from the solubilized biomass; and, (c) optionally the biofuel and/or chemical compound is separated from the composition.

Use of metal salts and deep eutectic solvents in a process to solubilize a biomass

Methods and compositions (e.g., engineered hosts) are disclosed for use in converting biomass to 3-hydropropionic acid. In particular embodiments, the methods include use of an engineeredyeast, such as, the engineeredhaving the RT04_8975 gene deleted from its genome, combined with a lignocellulosic hydrolysate, sourced, for example, from a biomass. A promoter for enhancing transport of 3HP is also incorporated by addition to thegenome, for example, by modified lithium acetate transformation.

Modified yeast microorganisms to increase yield of 3-hydropropionic acid

The present invention provides for a genetically modified fungal host cell capable of producing prespatane and/or epi-isozizaene comprising prespatane synthase (PPS) and/or epi-isozizaene synthase (EIZS).

Genetically modified fungal cells and methods useful for producing prespatane

Fungi that are genetically inactivated for the mstC gene (or a homolog thereof) are provided, which can also be genetically modified to increase production of heterologous proteins from a glucoamylase promoter. Methods of using these fungi, for example to degrade a biomass, are also provided.

Gene targets for improved enzyme production in fungi

The present disclosure relates to genetically engineered host cells and methods of producing a lipid-derived compound by employing such host cells. In particular embodiments, the host cell includes a first mutant gene encoding a cytoplasmic tRNA thiolation protein. Optionally, the host cell can include other mutant genes for decreasing fatty alcohol catabolism, decreasing re-importation of secreted fatty alcohol, or displaying other useful characteristics, as described herein.

Methods of producing lipid-derived compounds and host cells thereof

The present invention provides for anhost cell comprising a gene of interest operatively linked to an ecm33 promoter of an ascomycete fungi, wherein the gene of interest is heterologous to the ecm33 promoter and/or to. In some embodiments, the gene of interest is a glycoside hydrolase enzyme. In some embodiments, the glycoside hydrolase enzyme is a glucosidase.

Promoter useful for high expression of a heterologous gene of interest in

The present invention provides for a method of converting a depolymerized lignin aromatic compound into a bioproduct, comprising: (a) providing a composition comprising a depolymerized lignin aromatic compound, optionally a depolymerized cellulose, and optionally a depolymerized hemicellulose, and (b) introducing a genetically modified microorganism to the composition, wherein the genetically modified microorganism is capable of converting the depolymerized lignin aromatic compound into a bioproduct; such that the depolymerized lignin aromatic compound is converted into a bioproduct.

Synthesis of bioproducts from lignin-derived aromatics by genetically modified microorganisms

The present invention relates to terpene synthases capable of degrading precursors into biofuel compounds, such as terpenoid compounds. In one instance, a transformed organism can include such terpene synthases, as well as vectors encoding such synthases. Methods of employing such synthases and organisms are also described herein.

Terpene synthases for biofuel production and methods thereof

The present invention provides for a purified or isolated cellulase complex comprising two or more glycosidase hydrolase, or enzymatically active fragment thereof, selected from the group consisting of a GH9 polypeptide, a GH48 polypeptide, a GH10 polypeptide, and a GH6 polypeptide, and optionally a GH10_2 polypeptide and/or an AA10 polypeptide.

Cellulase complex, and glycosidase hydrolases thereof, and methods of using thereof

Disclosed herein are methods for preparing sugar compositions. The methods include: i) forming a mixture including polysaccharide biomass and an ionic liquid solution, wherein the ionic liquid solution contains water and an ionic liquid, and the ionic liquid contains a) a cation and b) a sugar acid anion or a ketoacid anion; ii) maintaining the mixture under conditions sufficient to dissolve at least a portion of the polysaccharide present in the polysaccharide biomass; iii) adding at least one glycoside hydrolase to the mixture; and iv) maintaining the mixture containing the glycoside hydrolase under conditions sufficient to hydrolyze at least a portion of the dissolved polysaccharide, thereby forming the sugar compositions. The sugar compositions contain at least one monosaccharide or oligosaccharide. New sugar-based ionic liquids are also described.

Conversion of sugars to ionic liquids

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Alameda, CA, United States of America

John Michael Gladden