Molecular Probes, Inc.

John Michael Brinkley

Richard P Haugland

Hee Chol Kang

Victoria L Singer

Michael A Kuhn

K Sam Wells

Yu Zhong Zhang

The invention is a novel fluorescently labeled microparticle, where the microparticle internally incorporates at least one dipyrrometheneboron difluoride dye. Appropriate selection of substituents results in dipyrrometheneboron difluoride derivatives that, when incorporated into polymer microparticles, give the desired excitation and emission wavelengths. The spectral characteristics of the labeling dyes in liquid are not greatly changed when the dye is incorporated into the particles, and the spectral excitation and emission wavelengths are compatible with commonly used filter sets. Other embodiments of the fluorescent microparticles include additional dyes and/or bioreactive substances.

Dipyrrometheneboron difluoride labeled flourescent microparticles

The invention relates to microparticles incorporating a series of two or more fluorescent dyes having overlapping excitation and emission spectra allowing efficient energy transfer from the excitation wavelength of the first dye in the series, transfer through the dyes in the series and re-emitted as an optical signal at the emission wavelength of last dye in the series, resulting in a desired effective Stokes shift which is controlled through selection of appropriate dyes.

Fluorescent microparticles with controllable enhanced stokes shift

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Elmira, OR, United States of America

John Michael Brinkley