Syngenta Participations Ag

University of California

Other

The General Hospital Corporation

The United States of America As Represented by the Secretary of

Commonwealth Scientific and Industrial Research Organisation

Greenvenus, LLC

John M Salmeron

Frederick M Ausubel

Cheryl Peters

Brian J Staskawicz

Thomas M Hohn

Fumiaki Katagiri

Barbara J Baker

Jeffrey Ellis

Guo-Liang Yu

Michael Nicholas Mindrinos

Douglas Dahlbeck

Andrew F Bent

Manuel B Sainz

Barbara N Kunkel

Giles Edward Oldroyd

Brian S Staskawicz

Caius Rommens

Zhongsen Li

Andrey A Boukharov

Robert Arthur Dietrich

Shiv Bahadur Tiwari

Stephen Ezra Schauer

Sekhar Boddupalli

Arianne Tremblay

Rio Stamler

Michael G Willits

Tesfaye Mengiste

Laura Jean Weislo

Xi Chen

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest 'on-demand' by treatment of the plant with a chemical ligand.

Control of phenotype in plants

The present invention pertains to an isolated promoter sequence fromBOSI gene encoding a protein for biotic and abiotic stress tolerance. Also, the invention relates to recombinant vectors, expression cassettes, host cells, plants or progeny thereof comprising nucleic acid molecules operably linked to said promoter.

[object Object]

The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) expressing the candidate plant disease-resistance gene within the plant tissue sample; and (d) determining whether the plant tissue sample exhibits a disease-resistance response, whereby a response identifies a plant disease-resistance gene.

Methods of identifying plant disease-resistance genes

The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.

Transgenic plants compromising nucleic acid molecules encoding RAR1 disease resistance proteins and uses thereof

The present invention discloses isolated polynucleotides encoding polypeptides having trichothecene 3-O-acetyltransferase activity, recombinant vectors and host cell comprising said polynucleotides.

Trichothecene-resistant transgenic plants

The present invention pertains to isolated nucleic acid molecules comprising nucleotide sequences isolated fromthat encode RARI proteins involved in disease resistance, and recombinant vectors comprising said nucleotide sequences.

Nucleic acid molecules from rice encoding RAR1 disease resistance proteins and uses thereof

The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (&ldquo;DAS&rdquo;), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (&ldquo;DON&rdquo;) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as or

Homologues of the Arabidopsis NIM1 gene, which is involved in the signal transduction cascade leading to systemic acquired resistance (SAR), are isolated from (tobacco), (tomato), (oilseed rape), (sugarbeet), (sunflower), and (potato). The invention further concerns transformation vectors and processes for expressing the NIM1 homologues in transgenic plants to increase SAR gene expression and enhance broad spectrum disease resistance.

Plant genes and uses thereof

Trichothecne-Resistant transgenic plants

Disclosed is substantially pure DNA encoding an,Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

RPS gene family, primers, probes, and detection methods

The Prf gene of tomato has been cloned and analyzed. Prf encodes a protein with leucine-rich repeat, nucleotide binding, and leucine zipper motifs, identifying it as a member of the resistance gene class that includes RPS2, RPM1, N and L6. When expressed in transgenic plants, Prf confers Fenthion sensitivity and resistance to a wide variety of phytopathogens, including not only,but also unrelated pathogens such as

Prf protein and nucleic acid sequences: compositions and methods for plant pathogen resistance

Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.

The Prf gene of tomato has been cloned and analyzed. Prf encodes a protein with leucine-rich repeat, nucleotide binding, and leucine zipper motifs, identifying it as a member of the resistance gene class that includes RPS2, RPM1, N and L6. When expressed in transgenic plants, Prf confers Fenthion sensitivity and resistance to a wide variety of phytopathogens, including not only Pseudomonas syringae but also unrelated pathogens such as Xanthomonas campestris.

Growing community of inventors

Hillsborough, NC, United States of America

John M Salmeron