Cephalon, Inc.

Chiron Corporation

Other

John M Hanson

Russell A Brierley

Francis C Maslanka

Joan N Abrams

Glenn J Dorin

Cynthia A Cowgill

Patricio T Riquelme

Luis Juarbe-Osorio

Carl Scandella

Christopher M Bussineau

Robert D Kudrna

Asuman G Ozturk

Methods for purifying authentic, properly folded IGF polypeptides from yeast transformants are disclosed. The methods include a refolding step and provide for high yields of authentic IGF from a variety of yeast strains.

Methods for purifying authentic IGF from yeast hosts

Methods of purifying authentic IGF from yeast hosts

Disclosed is an improved process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The improvements, consisting primarily of the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step result in a three-fold increase in final yield. The process includes the following steps, in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.

IGF-I purification process

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Encinitas, CA, United States of America

John M Hanson