Xy, Inc.

Chd Bioscience, Inc.

Colorado State University Research Foundation

Cdh Bioscience, Inc.

Chata Biosystems, Inc.

John Louis Schenk

George E Seidel

Lisa Ann Herickhoff

Thomas Boyd Gilligan

Edwin Dean Neas

Jerald Edward Kuiken

Tae Kwang Suh

Juan F Moreno

Maurice A Rosenstein

Allison C Lindsey

A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device.

Generating a fluid stream in a microfluidic device

Compositions () and methods of using such compositions () to condition, clean, or disinfect the flow path of a conduit () of a microfluidic devices (), such as flow cytometers or liquid chromatographs.

Antimicrobially active compositions

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

System and method for sorting cells

Flow path conditioner system

A sperm cell process system providing to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells.

Sperm cell separation system

A sex selected equine embryo production system for the production of sex selected equine embryos and offspring by fertilization of oocytes with sex selected sperm by intracytoplasmic sperm injection.

Sex selected equine embryo production system

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.

Heterogeneous inseminate system

The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.

Methods and apparatus for reducing protein content in sperm cell extenders

Low pressure sperm cell separation system

A method of continuously regulating external gas pressure on a variable volume container for the delivery of fluids to a microfluidic device and analysis of particles based upon relative presence or absence of at least one particle characteristic.

Method of generating a fluid stream in a microfluidic device

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

Method of cryopreserving selected sperm cells

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

Growing community of inventors

Fort Collins, CO, United States of America

John Louis Schenk