Hewlett-packard Development Company, L.P.

Oregon State University

John Lahmann

Pavel Kornilovich

Alexander N Govyadinov

Paul Mark Haines

Silam J Choy

Paul J Benning

Diane R Hammerstad

Si-lam Choy

Kenneth James Faase

Hilary Ely

Adam Z Higgins

Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

Microfluidic reaction chamber for amplification of nucleic acids

An example method includes providing fluid to a chamber. The chamber feeds a first channel terminating at a first droplet ejector and a second channel terminating at a second droplet ejector. The method further includes sequencing ejection of droplets at the first droplet ejector and the second droplet ejector to induce negative pressure to provide a sequenced output flow of the fluid through the first channel to a first target microfluidic network and through the second channel to a second target microfluidic network, and controlling the first and second target microfluidic networks to perform an analytical process with the fluid.

Sequenced droplet ejection to deliver fluids

A microfluidic valve can include a substrate having a microfluidic channel formed in the substrate. A sealing layer can be over the microfluidic channel. A flexible blister layer can be over the sealing layer. The flexible blister layer can include a blister formed as a distended portion with a blister volume between the flexible blister layer and the sealing layer. The microfluidic valve can be actuatable by puncturing the sealing layer by pressing on the blister. Actuating the microfluidic valve can either allow fluid to flow through the microfluidic channel or block fluid from flowing through the microfluidic channel.

Microfluidic valves

A microfluidic photoreactor for treating excess bilirubin in blood, having: a microfluidic channel module; an illumination module comprising one or more illumination sources disposed about the microfluidic channel module and configured to illuminate blood passing through at least one microfluidic channel of the microfluidic channel module; and a heat exchanger module coupled to the at least one microfluidic channel module, wherein the heat exchanger module is configured to extract heat from the at least one microfluidic channel. A system including a microfluidic photoreactor and a method of treating excess bilirubin in blood.

Microfluidic removal of excess bilirubin from blood

An example device includes a droplet ejector including a nozzle to eject droplets of a fluid and a target medium to receive the droplets of the fluid. The target medium is separated from the droplet ejector by a gap to be traversed by the droplets. The example device further includes a frame affixing the target medium to the droplet ejector. The target medium is immovably held with respect to the droplet ejector.

Droplet ejectors with target media

An example device includes a first droplet ejector including a first nozzle to eject droplets of a first fluid, and a first target medium positioned relative to the first droplet ejector to receive the droplets of the first fluid from the first droplet ejector. The example device further includes a second droplet ejector in fluid communication with the first target medium to receive a second fluid from the first target medium. The second droplet ejector includes a second nozzle to eject droplets of the second fluid.

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Corvallis, OR, United States of America

John Lahmann