Ribi Immunochem Research, Inc.

John L Cantrell

Edgar E Ribi

Steven M Schwartzman

Jon A Rudbach

Vaccines are provided which are composed of (a) non-toxic and highly effective adjuvants obtained from microbial sources, together with (b) tumor antigens. A wide variety of antigens can be employed in the vaccines and include, antigens obtained from tumors or cultures of tumor cells, such as ovarian cancers, melanomas, colorectal cancers, pancreatic cancers, renal cancers and the like. By adding tumor antigens to potent but non-toxic immunostimulants, a protective and lasting tumor immunity can be obtained.

Vaccine containing tumor antigens and adjuvants

An immunological adjuvant is provided which enhances the immune response against antigens, and accordingly is useful in vaccines. The adjuvant system is comprised of (1) a lipid emulsion system containing a metabolizable oil, a low molecular weight polyol and lecithin and (2) at least one refined detoxified bacterial biological adjuvant.

Immunological lipid emulsion adjuvant

An immunological adjuvant for polysaccharide vaccines is provided whereby polysaccharide antigens are rendered more immunogenic and thus stimulate more antibody production including an IgG response and immunological memory. The adjuvant is comprised of (1) either (A) an emulsion system containing a metabolizable oil, a low molecular weight polyol and lecithin, or (B) an oil-in-water emulsion system containing a light hydrocarbon non-biodegradable oil or a biodegradable oil, and a detergent; and (2) a refined detoxified endotoxin. The adjuvant can optionally also contain trehalose dimycolate.

Immunological emulsion adjuvants for polysaccharide vaccines

A pharmaceutical composition comprising a purified pyridine-soluble extract of a microorganism and a refined detoxified endotoxin which is effective in producing an immunological response in warm blooded animals and humans. Methods of using the composition for these purposes are also disclosed.

Pyridine-soluble extract-refined detoxified endotoxin composition and use

A purified pyridine-soluble extract of a microorganism is disclosed. The extract in a pharmaceutically acceptable medium is useful as an anti-animal tumor agent in the treatment of animals.

Pyridine-soluble extracts of microorganisms

A stable composition containing cell wall skeleton (CWS) and trehalose dimycolates (TDM) which may be effectively reconstituted after lyophilization. The composition is useful in the treatment of various cancerous tumors in animals without side effects. Also disclosed is a method of producing the composition and a method of treating cancerous tumors in animals using said composition.

Stable composition and preparation thereof

A pharmaceutical composition is disclosed comprising a purified pyridine-soluble extract obtained from a microorganism which contains between about 7 and 20% by weight of protein, between about 10 and 16% by weight of sugar, and between about 35 and 55% by weight of fatty acids which when combined with cell wall skeleton and trehalose dimycolate in a pharmaceutically acceptable medium is useful as an anti-animal tumor agent in the treatment of animals.

Pyridine soluble extract of a microorganism

A pharmaceutical composition comprising refined detoxified endotoxin, trehalose dimycolate, and an acetone precipitated by-product of endotoxic glycolipids extracted with chloroform-methanol (ACP) is disclosed which is useful for the treatment of cancerous tumors. Methods of using the composition for these purposes are also disclosed.

Refined detoxified endotoxin product

A pharmaceutical composition comprising refined detoxified endotoxin, cell wall skeleton and trehalose dimycolate is disclosed which is effective for the treatment of cancerous tumors. Methods of using the composition for these purposes are also disclosed.

A pharmaceutical composition is disclosed comprising a purified pyridine-soluble extract obtained from microorganism which contains between about 7 and 20% by weight of protein, between about 10 and 16% by weight of sugar, and between about 35 and 55% by weight of fatty acids which when combined with trehalose dimycolate and an acetone precipitated by-product of endotoxic glycol lipids extracted with chloroform-methanol in a pharmaceutically acceptable medium is useful as an anti-animal tumor agent in the treatment of animals.

A method of producing a purified pyridine-soluble extract of a microorganism is disclosed which contains between about 7 and 20% by weight of protein, between about 10 and 16% by weight of sugar, and between about 35 and 55% by weight of fatty acids. The extract when combined with cell wall skeleton in a pharmaceutically acceptable medium is useful as an anti-tumor agent in the treatment of animals.

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Hamilton, MT, United States of America

John L Cantrell