Visible Genetics Inc.

Other

Ebox Inc.

Visible Assets, Inc.

Hsc Research and Development Limited

Ebox USA Inc.

John K Stevens

James M Dunn

Paul Waterhouse

John A Renfrew

Alexandre M Izmailov

Marina T Larson

Bruno C Maruzzo

Thomas D Yager

Paul I Waterhouse

Gregory Dee

Ronald J Green

Eleftherios P Diamandis

Henryk Zaleski

May Hui

Rodney D Gilchrist

Vrijmoed Chi

Brenda L Gallie

Alexandre M Ismailov

Denis Capatos

David E Matthews

James Leushner

James W Cassidy

Eric Steinbach

A method for testing the installation of a box includes the steps of closing the lid of the box, transmitting a signal from the box; noting the absence of an annunciation; and variously repositioning the box relative to a receiver, replacing a battery, or replacing the box.

Testing methods for use with boxes

Openings and closings of a secured storage receptacle ( ) are logged to record the transfer of items. The system gains access to a signal-transmitting receptacle; transmits a first confirmation signal to a mobile transmission node ( ); processes the first confirmation signal at the mobile transmission node ( ); transmits a second confirmation signal to a central server ( ); and notifies a consumer of the delivery with an electronic message ( ). This process allows the transfer of items by a delivery confirmation signals to be received from the signal-transmitting receptable ( ). The signal transmitted from the signal-transmitting receptable ( ) is coupled to the opening and closing of the storage box.

Computerized recording and notification of the delivery and pickup of retail goods

A package storage and delivery system includes electronically controlled lockers disposed at or near customer locations. Each locker is unlocked by a courier, preferably by means of a short-range transceiver or transmitter carried on the courier's person. The customer can unlock the locker and receive the delivered package. Cryptographically signed communications are employed along with nonvolatile usage logs to minimize the risk of loss of a package or fraud by courier or customer. The lockers may be stackable, permitting a delivery courier to add lockers in the event a customer receives too many deliveries to fit into a single locker. Each box has, of course, a physical location, and has associated with it an address code indicative of the physical location, for example by means of a human-readable or compressed representation of the precise latitude and longitude. A package delivered to such a box preferably bears the address code. A merchant can greatly reduce the risk of credit card fraud by requiring the use of such codes for the simple reason that a fraudulent transaction may be traced to a specific physical location.

Package delivery system

Normalization of experimental fragment patterns for nucleic acid polymers having putatively known sequences starts with obtaining at least one raw fragment pattern for the experimental sample. The raw fragment pattern represents the positions of a selected nucleic acid base within the polymer as a function of migration time or distance. This raw fragment pattern is conditioned using conventional baseline correction and noise reduction technique to yield a clean fragment pattern. The clean fragment pattern is then evaluated to determine one or more &ldquo;normalization coefficients.&rdquo; These normalization coefficients reflect the displacement, stretching or shrinking, and rate of stretching or shrinking of the clean fragment, or segments thereof, which are necessary to obtain a suitably high degree of correlation between the clean fragment pattern and a standard fragment pattern which represents the positions of the selected nucleic acid base within a standard polymer actually having the known sequence as a function of migration time or distance. The normalization coefficients are then applied to the clean fragment pattern to produce a normalized fragment pattern which is used for base-calling in a conventional manner. This method may be implemented in an apparatus comprising a computer processor programmed to determine normalization coefficients for an experimental fragment pattern. This computer may be separate from the electrophoresis apparatus, or part of an integrated unit.

Method and system for DNA sequence determination and mutation detection

A hierarchy of at least two assay techniques is utilized in testing for disease-associated mutations. The first assay in the hierarchy is selected to provide a highly specific test for the existence of the disease-associated mutation, although the accuracy of the test need not be high. The final assay in the hierarchy is selected to provide a highly accurate and highly specific test for the existence of the disease associated mutation. Intermediate tests of progressively greater accuracy may also be included in the hierarchy. Once the hierarchy has been selected for a given mutation-associated disease, a patient sample is analyzed the patient sample using the first, lowest accuracy assay in the hierarchy. If the result of the first assay is negative for the presence of a disease-associated mutation, then the next assay in the hierarchy is performed. This process is repeated until the final assay has been performed on all samples which gave negative results when tested by all less-accurate assays in the hierarchy. The test may be used for diagnosis and targeted screening for p53 mutations and mutations in the RB1 gene.

Method for testing for mutations in DNA from a patient sample

A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 &mgr;m wide and 1 to 10 &mgr;m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric filed within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

Micro-electrophoresis chip for moving and separating nucleic acids and other charged molecules

A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 &mgr;m wide and 1 to 10 &mgr;m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

Nanofabricated separation matrix for analysis of biopolymers and methods

Method, reagents and kit for diagnosis and targeted screening for p53

Reliable and cost effective testing for mutations in the RB1 gene can be accomplished by (a) quantitatively amplifying exons of the sample RB1 gene using primers complementary to intron regions flanking each exon; and (b) determining the lengths and/or quantities of the amplification products for each exon and comparing that length or quantity to the length or quantity of amplification products obtained when a wild-type RB1 gene is amplified using the same primers. Differences in length between an amplified sample exon and the corresponding amplified wild-type exon reflect the occurrence on an insertion or deletion mutation in the sample RB1 gene. Differences in quantity reflect the complete absence of an exon, or heterozygosity for a mutant exon. Next, the nucleic acid sequence of each exon found to contain an insertion or deletion mutation is determined, or of all exons in the event no insertion or deletion mutations are identified. Preferably, the amplification of the exons is multiplexed so that more than one exon is amplified in a single vessel using sets of primers which provide gene fragments of distinctive lengths when used to amplify a normal RB1 gene.

Method, reagents and kit for diagnosis and targeted screening for

The instant invention relates to methods and products for the diagnosis of mutations in the von Hippel-Lindau tumor suppressor gene. More specifically, the products are DNA oligonucleotides for use in amplifying and sequencing genomic DNA and kits including these oligonucleotides. The method of the invention relates to a hierarchical system for cost-effective diagnosis of von Hippel-Lindau tumor suppressor disease-associated mutations.

Compositions and methods for diagnosis of mutation in the von

Apparatus for preparing gels for use in electrophoretic separations and

Automated electrophoresis and fluorescence detection apparatus and method

Changes in polarized light incident on a detection zone within a separation matrix are used to detect optically active molecules within the separation matrix. The separation and detection of optically active molecules within the detection zone is done by loading a sample containing optically active molecules onto a separation matrix; applying a motive force to cause the sample to migrate though the separation matrix and to separate into a plurality of subgroups of optically active molecules; directing an incident beam of polarized radiation to the detection zone; processing the collected exiting beam with an optical component which discriminates between radiation having the same polarization as the incident beam and radiation having a different polarization from the incident beam; and measuring the intensity of the processed exiting beam. A difference between the intensity of the processed exiting beam and a standard intensity obtained for the separation matrix in the absence of sample is indicative of the presence of an optically active molecule in the detection zone. Alternatively, an incident beam of alternately left and right circularly polarized radiation, in which case differences in the intensity of left and right circularly polarized radiation indicate the present of optically acid species within the detection zone.

Method and apparatus for automated electrophoresis using light

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Toronto, Canada

John K Stevens