Signature Bioscience, Inc.

Other

John J Hefti

Mark A Rhodes

Barrett Bartell

Dean M Drako

Kurt Joseph Kramer

Robert G Chapman

Hong Peng

Tyler A Palmer

Min Zhao

A system operable to monitoring bio/chemical activities includes a first measurement probe, a second measurement probe and a comparator. The first measurement probe is operable to interrogate one or more physical properties of a sample at a first location of the sample, and to output, in response, a first measurement signal. The second measurement probe is operable to interrogate one or more physical properties of the sample at a second location of the sample, and to output, in response, a second measurement signal. The comparator is coupled to receive the first and second measurement signals, the comparator configured to output a difference signal comprising the difference between the first and second measurement signals, the difference signal corresponding to the difference in one or more bio/chemical activities occurring at the first location of the sample relative to the second location of the sample.

Systems and methods for monitoring chemical and biological activities using differential measurements

A coplanar waveguide biosensor and methods of use include a coplanar waveguide transmission line and a sample containment structure. The coplanar waveguide transmission line is operable to support the propagation of an electromagnetic signal and includes a signal line and one or more spaced apart ground elements. The signal line is configured to conduct a time-varying voltage, and the one or more ground elements are configured to maintain a time-invariant voltage, a detection region being formed between a portion of the signal line and a portion of at least one of the one or more ground elements. Detection methods are improved through the enhancement of the electric field in the detection region via impedance discontinuities in the signal line and ground elements. The sample containment structure intersects the detection region of the coplanar waveguide transmission line and includes a cavity configured to hold 1 ml or less of sample solution within the detection region.

Coplanar waveguide biosensor for detecting molecular or cellular events

The present invention provides a method for detecting a molecular event, comprising (1) applying an electromagnetic test signal to a sample in which a molecular event is being detected, whereby the sample interacts with and modulates the test signal to produce a modulated test signal, and (2) detecting the modulated test signal, wherein the applying and detecting take place in a temperature-controlled environment, wherein the temperature-controlled environment comprises the sample, a radiating portion of a signal generating circuit, and a receiving portion of a signal detection circuit and wherein the applying and detecting take place in the environment at a temperature controlled to within &plusmn;0.5&deg; C.

Method and apparatus for detection of molecular events using temperature control of detection environment

A system and method for detecting a molecular event in a test sample using a resonant test structure is presented. The method includes providing a resonant test structure having a resonant response associated therewith. Next, a first resonant response of the resonant test structure is obtained when the resonant test structure is electromagnetically coupled to a reference sample, the reference sample having a known composition. A second resonant response is also obtained when the resonant test structure is electromagnetically coupled to the test sample, the test sample having an unknown composition. Subsequently, one or more first electrical parameters, such as the q-factor of the resonator, are derived from the first resonant response. One or more second electrical parameters are similarly derived from the second resonant response. The similarity or difference between the first and second electrical parameters are analyzed to determine the presence or absence of the molecular event in the test sample.

System and method for detecting and identifying molecular events in a test sample using a resonant test structure

The present invention provides a variety of methods of analyzing protein binding events using a system capable of directly detecting protein/ligand complexes based upon the dielectric properties of the complex. The system can be used in a variety of analyses involving protein binding events, such as screening ligand libraries, characterizing protein binding interactions, and identifying ligands. The system can also be utilized in diverse analytical and diagnostic applications.

Methods for analyzing protein binding events

A bio-assay test system includes a test fixture, a measurement system, and a computer. The test fixture includes a bio-assay device having a signal path and a retaining structure configured to place a sample containing molecular structures in electromagnetic communication with the signal path. The measurement system is configured to transmit test signals to and to receive test signals from the signal path at one or more predefined frequencies. The computer is configured to control the transmission and reception of the test signals to and from the measurement system.

Bio-assay device

Systems and methods for detecting molecular binding events and other environmental effects using the unique dielectric properties of the bound molecular structure or structures are presented. A molecular binding layer is coupled along the surface of a signal path. A test signal is propagated along the signal path, whereby the test signal couples to the molecular binding layer, and in response, exhibits a signal response.

Computer program and database structure for detecting molecular binding events

Systems and methods are presented for detecting molecular binding events and other environmental effects using the unique dielectric properties of the bound molecular structure or structures. A molecular binding region is coupled along the surface of a signal path. A test signal is propagated along the signal path, whereby the test signal couples to the molecular binding region, and in response, exhibits a signal response.

Resonant bio-assay device and test system for detecting molecular binding events

Bio-assay device and test system for detecting molecular binding events

The present invention provides a variety of methods for conducting analyses of nucleic acids using a detection system which is sensitive to the unique dielectric properties of different hybridization complexes and which can distinguish between different hybridization complexes directly without the use of labels. Methods using the system to perform sequence checking, expression analysis, de novo sequencing and a variety of other nucleic acid analyses are provided.

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San Francisco, CA, United States of America

John J Hefti