Cargill Inc.

John J Hahn

Timothy Walter Abraham

Paraskevas Tsobanakis

Erik Allen

Christopher Lawrence Frank

Eric Christopher Bohnert

Troy Allen Rhonemus

Christopher Austin Tyler

Allan S Myerson

Jeffrey C Evans

Andrew Keith Ohmes

Kern M Storo

Ronny Leontina Marcel Vercauteren

An Amanda Jules Heylen

Alexander Patist

Aron Broman Erickson

Chen-Chou Chiang

Jenna Stangler

Timothy Oolman

Alan Stuart Myerson

A method for recovering a composition enriched in 3-hydroxypropionic acid from a fermentation broth comprising 3-hydroxypropionic acid and/or salts thereof comprises the steps of: (a) providing the fermentation broth having a pH of from about 2 to about 8 comprising: 3-hydroxypropionic acid and/or salts thereof, (b) acidifying the fermentation broth; (c) reducing the total sulfate ion and phosphate ion (d) distilling the resulting reduced ion aqueous solution and (e) recovering the product.

Recovery of 3-hydroxypropionic acid

A method for recovering a composition enriched in 3-hydroxypropionic acid by providing the fermentation broth, acidifying the fermentation broth; reducing the total sulfate ion and phosphate ion concentration of the resulting aqueous solution to produce a reduced ion aqueous solution; distilling the resulting reduced ion aqueous solution and recovering the resulting aqueous distillation product comprising 3-hydroxypropionic acid.

Recovery of 3-hydroxpropionic acid

A method for recovering a composition enriched in 3-hydroxypropionic acid from a fermentation broth comprising 3-hydroxypropionic acid and/or salts thereof comprises the steps of: (a) providing the fermentation broth having a pH of from about 2 to about 8 comprising 3-hydroxypropionic acid and/or salts thereof (b) acidifying the fermentation broth; (c) reducing the total sulfate ion and phosphate ion (d) distilling the resulting reduced ion aqueous solution and (e) recovering the product.

The present invention provides methods to treat mixtures containing natural rebaudioside A (Reb A), rebaudioside B (Reb B), and rebaudioside D (Reb D), synthetic counterparts of these, and/or derivatives of the natural or synthetic embodiments obtain one or more of these glycosides in more pure form. In many embodiments, the invention can be used to process glycoside mixtures obtained at least in part from natural sources such as theplant. This allows, for instance, the recovery of a product including Reb A material in more pure form relative to Reb B material or Reb D material. As an alternative or in addition to recovery of the purified Reb A material, a product including Reb B material and/or Reb D material in more pure form relative to Reb A material can be obtained.

Methods to treat mixtures of glycosides to obtain one or more of these glycosides in more pure form

Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

Separation of rebaudioside A from stevia glycosides using chromatography

The invention provides methods of purifying rebaudioside A from a mixture comprising glycosides of the plant. The methods of the invention are useful for preparing highly pure rebaudioside A compositions from crudestarting compositions that are typically considerably lower in rebaudioside A concentration. The highly pure rebaudioside A compositions are useful as non-caloric sweeteners in edible or chewable compositions such as food, beverages, medicine, candy, chewing gum, and the like.

Method of producing purified rebaudioside A compositions using solvent/antisolvent crystallization

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Apple Valley, MN, United States of America

John J Hahn