Abbott Laboratories Corporation

Life Technologies Corporation

Invitrogen Corporation

Nanogen/becton Dickinson Partnership

Becton Dickinson and Company Limited

Nanogen, Inc.

John J Carrino

James Fan

Knut R Madden

Martin Anthony Gleeson

John A Salituro

Jonathan D Chesnut

James L Hartley

Michael A Brasch

Louis Leong

Ronald L Marshall

David Cheo

Gary F Temple

Edward K Pabich

Matthew T Manlove

John D Burczak

Paul A Klonowski

Devon R Byrd

Carl Frederick Edman

Michael I Nerenberg

Natalie A Solomon

Keith C Backman

Robert Bennett

Lorelei P Westin

Jonathan M Diver

Thomas G Laffler

Laurie A Rinehardt

Louis O Gerrue

Edward N Granados

Larry G Birkenmeyer

Jon D Kratochvil

Scott E Castanon

Brian Patrick Dwyer

Robert P Bennett

Richard R Anderson

Stanley R Bouma

Bruce J Dille

Robert R Yocum

Jorge Haro

Devon R N Byrd

Hsiang-Yun Y Hu

Uwe Spies

Jonathan Chestnut

Bryan G Towns

Joann C Sustachek

George H Shimer, Jr

Gray F Temple

Devon Rn Byrd

Martin A G Gleeson

Thomas D Brainard

Sheila B Bond

Martin J Bouliane

Gregory K Bond

John R Ertl

Paul M Jung

A method for using a single sample suspected of containing a microorganism for both a local rapid test immunoassay and a remote laboratory test. The sample is collected from a patient at a physician's office or from the environment to be tested. The sample is collected using a swab or other implement, combined with a rapid test processing reagent and a portion of the processed sample is used for the local rapid test. The rapid test processing reagent typically consists of a buffer, a salt, and a detergent and is compatible with the local rapid test immunoassay. Only a portion of the processed sample is used for the local rapid test, leaving a remaining portion of the processed sample to be used in a remote laboratory assay. At least some of the remaining portion of the processed sample is combined with a stabilization agent that preserves at least the nucleic acid in the processed sample for the remote laboratory assay.

Method for linking point of care rapid diagnostic testing results to laboratory-based methods

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

Methods and compositions for generating recombinant nucleic acid molecules

Cassette for a medical device

The method provided herein is a method for detecting a target sequence in a test sample. Generally, the method comprises forming a reaction mixture comprising a test sample, amplification reagents, a first primer, and a second primer wherein the concentration of the first primer in the reaction mixture is 15% to 250% percent greater than the concentration of the second primer. The target sequence is amplified according to any amplification protocol that employs the primer sequences to generate copies of the target sequence that include a product from the first and second primers. A probe is hybridized to the amplification product from the first primer to form a hybrid complex; and the hybrid complex is detected as an indication of the presence of the target sequence in the test sample.

Method for using unequal primer concentrations for generating nucleic acid amplification products

Compositions and methods for rapidly generating recombinant nucleic acid molecules

This invention relates to devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using ligation-dependent strand displacement amplification technologies in combination with bioelectronic microchip technology.

Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification an bioelectronic chip technology

A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.

Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures

Provided herein is a method for detecting the presence of a target nucleic acid sequence in a sample. The present method uses a combination of dual labeled probes and heterogeneous detection methods.

Analyte detection process using dual labeled probes

Growing community of inventors

San Diego, CA, United States of America

John J Carrino