Other

Commonwealth Scientific and Industrial Research Organisation

Bayer Cropscience Ag

Value Added Wheat Crc Limited

John Howard Skerritt

Stephen Charles Trowell

Amanda Susan Hill

Kim Astrid Garsia

Amanda S Hill

David P McAdam

Ian John Wesley

Gabriele Annierose Lang

Nigel Grant Larsen

Gabriele Annerose Lang

Brian George Osborne

Monoclonal antibody that specifically binds to alpha-amylase and is useful for detecting weather damage (i.e. pre-harvest sprouting) in cereal grain.

Monoclonal antibodies to alpha-amylase

A two-site immunoassay for the qualitative or quantitative detection of alpha-amylase in a test sample, said immunoassay comprising: (1) exposing said test sample to a first ('capture') antibody or fragment thereof which specifically or preferentially binds to a first epitope on said alpha-amylase, under conditions permitting binding of said first antibody or fragment thereof to alpha-amylase if present, (ii) subsequently exposing bound alpha-amylase, if any, to a second ('detection') antibody or fragment thereof which specifically or preferentially binds to a second epitope on said alpha-amylase that is distinct from said first epitope, under conditions permitting binding of said second antibody or fragment thereof to said bound alpha-amylase, and (iii) detecting any binding of said second antibody or fragment thereof to said bound alpha-amylase, wherein either of said first or second epitopes is an epitope comprising one or more of the amino acid sequences: IDRLVSIRTRGQIHS (SEQ ID NO:1), CRDDRPYADG (SEQ ID NO:2), VNWVNKVGGS (SEQ ID NO:3) and variants thereof showing &#x2267;80%, more preferably &#x2267;90%, sequence identity. The immunoassay is useful for detecting weather damage (i.e., preharvest sprouting) in cereal grain.

Detection of preharvest sprouting in cereal grains

The present invention provides an isolated immunoreactive molecule (IRM) capable of binding to a target molecule from at least one species or sub-species of the insect but not to at least one other species or sub-species of insect and a method of identifying one or more insect species or subspecies and kits therefor. The invention is particularly useful for distinguishing between Lepidopterans such as and and and

Agents and method for identifying insects

The present invention relates to a rapid and non-evasive method of monitoring dough development and dough mixing properties using near infrared (NIR) spectroscopy. In particular, the present invention relates to a method of measuring the change in constituents of a grain flour dough during mixing.

Monitoring of dough properties

An isolated immunoreactive molecule (IRM) capable of binding to a target molecule from at least one species or sub-species of the insect but not to at least one other species or sub-species of insect and a method of identifying one or more insect species or subspecies and kits therefor. The invention is particularly useful for distinguishing between Lepidopterans such as Helicoverpa armigera and H. punctigera.

Monoclonal and polyclonal antibodies and fragments thereof are described which are capable of binding to specific members or groups of O,O-dialkyl-O-(X) phosphorothioate compounds, where X is an aromatic or heterocyclic group or substituted aromatic or substituted heterocyclic group. Specifically, organophosphorus pesticides (e.g. fenitrothion, chloryrifos-methyl, and pirimiphos-methyl) are conjugated to a carrier with a beta-alanine spacer and used as an immunogen for raising antibodies. Hybridoma cell lines, methods for making the antibodies, and assays and kits including the antibodies are also disclosed.

Growing community of inventors

Cook, Australia

John Howard Skerritt