Bio-rad Laboratories, Inc.

John Healy

Sepehr Kiani

Tony Hung

Pascaline Mary

Adnan Moez Esmail

Tal Raz

Methods, libraries, and kits for nucleotide sequencing are provided. For example, methods can comprise providing a plurality of partitions, the partitions comprising a set of two or more different primers and a target nucleic acid, wherein different partitions contain different sets of primers with a majority of primers in the sets being common with a different primer set but wherein any two different partitions having a common primer contain no more than one common primer; and hybridizing the target nucleic acid to the primers in the reaction partitions under conditions in which fully complementary primers hybridize to the target sequence and primers that are not fully complementary do not hybridize to the target nucleic acid.

Systems and methods for sequencing in emulsion based microfluidics

A method for determining a sequence of a target nucleic acid is described. The method uses a plurality of control oligonucleotides with known sequence and unique identifications to map hybridization signals associated with a plurality of sequencing probes to a loosely packed multi-dimensional dye space, such that a region in the dye space can be associated with one or more sequencing probes. When a detected target hybridization signal of a sequencing probe and a target nucleic acid is mapped to the multi-dimensional dye space, the sequencing probe and thus the corresponding nucleotides in the target nucleic acid can be determined based on the one or more sequencing probes associated with the region that the detected target hybridization signal is mapped to.

Sparse identity spaces in droplet sequencing

The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

Integrated microfluidic system, method and kit for performing assays

Methods, libraries, and kits for nucleotide sequencing are provided.

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Acton, MA, United States of America

John Healy