Life Technologies Corporation

454 Life Sciences Corporation

Cyvek, Inc.

Roche Molecular Systems, Inc.

Quantum-si Incorporated

454 Lice Sciences Corporation

John Harris Leamon

Jonathan Marc Rothberg

Todd Rearick

Wolfgang Hinz

Mark R Andersen

Michael Thornton

Michael Weiner

Kenton L Lohman

James M Bustillo

Jonathan M Schultz

Jan Berka

Yi-Ju Chen

Maithreyan Srinivasan

Vinod B Makhijani

Kim L Johnson

John F Davidson

David Light

Martin A Putnam

John R Nobile

Michael Egholm

David Marran

Todd Roswech

Jon A Hoshizaki

Mark James Milgrew

Kristopher Barbee

Steven M Lefkowitz

Charles O Stanwood

Jeffrey T Branciforte

Sean McCusker

Ryan Jones

John W Simpson

Jonathan C Schultz

Melville Davey

Jonathan O Schultz

Gary James Sarkis

Jonathan M Rothberg

Antoine Van Oijen

Steve Lefkowitz

Martin Huber

Maximilian Carpino

Keith McDade

Jan Fredrik Simons

Brian Christopher Godwin

Joseph M Fierro

Xiaoxiao Ma

Greg A Volkmer

Bradley Hann

Maithreyn Srinivasan

William L Lee

Zhoutao Chen

James R Knight

Jaran Charumilind

Stephen Kyle Hutchison

Eugene W Myers, Jr

Michael T Ronan

Mike Todd Ronan

Jan Fredrick Simons

James Drake

Brian Desany

William Lee

Antoine M Van Oijen

Sean Mccusker

William Lun Lee

Michael P Weiner

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

Scaffolded nucleic acid polymer particles and methods of making and using

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Methods and apparatus including array of reaction chambers over array of chemFET sensors for measuring analytes

Embodiments described herein generally relate to apparatuses, cartridges, and pumps for peristaltic pumping of fluids and associated methods, systems, and devices. The pumping of fluids is, in certain cases, an important aspect of a variety of applications, such as bioanalytical applications (e.g., biological sample analysis, sequencing, identification). The inventive features described herein may, in some embodiments, provide an ability to pump fluids in ways that combine certain advantages of robotic fluid handling systems (e.g., automation, programmability, configurability, flexibility) with certain advantages of microfluidics (e.g., small fluid volumes with high fluid resolution, precision, monolithic consumables, limiting of the wetting of components to consumables).

Peristaltic pumping of fluids and associated methods, systems, and devices

Integrated sensor arrays for biological and chemical analysis

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Methods and compositions for multiplex PCR

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Methods and apparatus for measuring analytes using large scale FET arrays

ChemFET sensor array including overlying array of wells

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Bead emulsion nucleic acid amplification

A sample preparation apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. In addition, the sample preparation apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles, as well as a centrifuge for loading such particles onto a sensor array. The sample preparation apparatus can further include receptacles for holding containers of reagent solutions.

Centrifuge with variable angle buckets and method for loading a device

A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent. In some embodiments, the network may have at least two microfluidic channels, each channel of the two channels arranged to receive portions of the same fluid and to be fluidicly isolatable from each other, the device having at least two micro-particles disposed in fixed position in the network channels, the micro-particles being functionalized with a capture agent, one of the micro-particles in one of the channels being functionalized with an antibody or antigen capture agent and another of the micro-particles in another of the channels being functionalized with a nucleic acid capture agent. The method may also include detecting both protein and nucleic acid present in an input sample using the respectively functionalized micro particles. In some embodiments, the micro particles may be micro-length tubes or glass nano reactors.

PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use

Growing community of inventors

Guilford, CT, United States of America

John Harris Leamon